Ab212188

Manufactured by Abcam

Sourced in United States

Ab212188 is a high-quality primary antibody product manufactured by Abcam. It is designed for use in various laboratory applications. The core function of this product is to serve as a specific detection reagent for the target antigen.

Automatically generated - may contain errors

Lab products found in correlation

Ab92312, by Abcam (2 mentions) Ab110638, by Abcam (2 mentions) Ab92471, by Abcam (2 mentions) Ab200828, by Abcam (2 mentions) Ab109185, by Abcam (2 mentions) Ab207718, by Abcam (2 mentions) Ab3982, by Abcam (2 mentions) Ab15580, by Abcam (2 mentions) Ab137415, by Abcam (1 mentions) Ab6721, by Abcam (1 mentions) Ab9485, by Abcam (1 mentions) Ab182733, by Abcam (1 mentions) Ab182858, by Abcam (1 mentions) Anti rabbit igg as014, by ABclonal (1 mentions) Ab2302, by Abcam (1 mentions) Cl 0283, by Procell (1 mentions) M01263 2, by Boster Bio (1 mentions) Cp h058, by Procell (1 mentions) Ab288369, by Abcam (1 mentions)

4 protocols using ab212188

Western Blot Analysis of Cell Signaling Proteins

Cited 1 time

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Western blot assay was performed according to a previous protocol.^{25 (link)}Antibodies were hMSH2 (Abcam, ab212188, 1:1,000), E2F1 (Abcam, ab137415,
1:1,000), GAPDH (Abcam, ab9485, 1:2,500), and the anti-rabbit secondary antibody
(Abcam, ab6721, 1:2,000). The GAPDH was used as the loading control.

Hu X.Q., Zhang B.Y, & Hua T. (2023). hMSH2 coordinated with the expression of E2F1 promotes platinum response in epithelial ovarian cancer. The Journal of International Medical Research, 51(3), 03000605231163780.

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Antibody Profiling for Cancer Markers

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The following antibodies were purchased from the respective suppliers: anti-RNPS1 (ab79233), anti-Ki-67 (ab15580), anti-CEA (ab207718), anti-CA199 (ab3982), anti-CA153 (ab109185), anti-HE4 (ab200828), anti-Bcl-2 (ab182858), anti-Bax (ab182733), anti-MLH1 (ab92312), anti-cleaved caspase-3 (ab2302), anti-MSH2 (ab212188), anti-MSH6 (ab92471), and anti-PMS2 (ab110638) (all from Abcam, Cambridge, USA) and anti-β-actin (M01263-2; Boster, Wuhan, China). The secondary antibodies used were anti-rabbit IgG (AS014) and anti-mouse IgG (H+L) (AS003), both from ABclonal (Wuhan, China), and IMR-1A (HY-100431A; MedChemExpress, Dallas, TX, USA).

Liu X., Ma H., Ma L., Li K, & Kang Y. (2021). RNA-binding protein with serine-rich domain 1 regulates microsatellite instability of uterine corpus endometrial adenocarcinoma. Clinics, 76, e3318.

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Endometrial Cell Line Characterization

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This study used normal endometrial cells (NECs), KLE cells, RL952 cells, Ishikawa cells, and ECC-1 cells. They were maintained and cultured as previously described [15 (link)]. The cell lines used in this study were as follows: normal endometrial cells (NEC, CP-H058, Procell, Wuhan, China), KLE (CL-0133, Procell, Wuhan, China), RL952 (CL-0197, Procell, Wuhan, China), Ishikawa (CL-0283, Procell, Wuhan, China), and ECC-1 (BS-C163325, BinSuiBio, Shanghai, China).
The following antibodies were used: Anti-METTL5 (16791-1-AP, Proteintech, Rosemont, USA), anti-Ki-67 (ab15580, Abcam, Cambridge, USA), anti-CEA (ab207718, Abcam, Cambridge, USA), anti-CA199 (ab3982, Abcam, Cambridge, USA), anti-CA153 (ab109185, Abcam, Cambridge, USA), anti-HE4 (13, ab200828, Abcam, Cambridge, USA), anti-MLH1 (ab92312, Abcam, Cambridge, USA), anti-MSH2 (ab212188, Abcam, Cambridge, USA), anti-MSH6 (ab92471, Abcam, Cambridge, USA), anti-PMS2 (ab110638, Abcam, Cambridge, USA), and anti-β-actin (M01263-2, Boster, Wuhan, China).

Liu X., Ma H., Ma L., Li K, & Kang Y. (2022). The potential role of methyltransferase-like 5 in deficient mismatch repair of uterine corpus endometrial carcinoma. Bioengineered, 13(3), 5525-5536.

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Evaluating E2F1 and hMSH2 Expression

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In total, 36 tissue samples were obtained from the 77 patients and
immunohistochemistry (IHC) staining was performed for hMSH2 and E2F1 using the
hMSH2 antibody (ab212188, Abcam, 1:10000) and E2F1 antibody (ab288369, Abcam,
1:50). Nuclear staining without cytoplasmic staining was considered positive.
E2F1 and hMSH2 protein expressions were evaluated based on previous
studies.^{23 (link),24 (link)} Specifically, staining intensity and percentage of
positive staining area of the IHC results were set as judgment criteria.

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