Total RNA was isolated from bone marrow and peripheral blood samples using Trizol reagent (Life Technologies Carlsbad, CA, USA). cDNA was synthesized using SuperScript III reagents (Life technologies) according to the manufacturer's instructions. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using commercially available TaqMan Gene Expression Assay primers and probes for SOD2 and B2M and the LightCycler 480II Real-Time PCR System (Roche, Basel, Switzerland). The expression levels were normalized to B2M gene expression.
Taqman gene expression assay primers and probes
Manufactured by Roche
Sourced in Switzerland
The TaqMan Gene Expression Assay primers and probes are laboratory equipment designed for the detection and quantification of gene expression. They provide a standardized and reliable method for analyzing the presence and levels of specific RNA or DNA sequences in a sample.
Automatically generated - may contain errors
2 protocols using taqman gene expression assay primers and probes
1
Quantifying SOD2 Expression in Hematopoietic Cells
2
Quantifying SOD2 Expression in Hematopoietic Cells
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