Crl cd sd female rats

Male transgenic rats (SD-Tg(CAG-EGFP)4Osb) were purchased from Slc Japan (Shizuoka, Japan) (http://www.anim.med.kyoto-u.ac.jp/NBR/strains/Strains_d.aspx?StrainID=559&s_geneAffected=GFP&s_References=GFP&s_livingAnimals=1) and were used as sperm donors at 11–12 weeks of age; Slc:SD female rats (purchased from Slc) were used as oocyte donors at 4–5 weeks of age. Meanwhile, Crl:CD (SD) female rats purchased from Charles River Japan (Kanagawa, Japan) were used as recipients of fertilised oocytes at 10–15 weeks of age. All animals were housed under a twelve-hour dark/light cycle (light from 07:00 to 19:00) at 22 °C ± 1 °C, with ad libitum access to food and water. The Animal Care and Use Committee of Kumamoto University School of Medicine approved the protocols for animal experiments and all methods were performed in accordance with the relevant guidelines and regulations.

Jcl:SD rats were purchased from CLEA Japan Inc. (Tokyo, Japan) to use as sperm and oocyte donors. Sperm were obtained from male rats (11–15 weeks old) and oocytes were obtained from immature female rats (5–6 weeks old). Crlj:CD(SD) male rats (> 13 weeks old) were purchased from Charles River Japan (Kanagawa, Japan) to use as vasectomised male rats for embryo transfer. Crl:CD(SD) female rats (10–12 weeks old) were purchased from Charles River Japan to use as recipients for embryo transfer. Male transgenic rats (SD-Tg(CAG-EGFP)4Osb) and female rats (Slc:SD) purchased from Slc Japan (Shizuoka, Japan) were used for cold transport. The breeding environments were light (07:00–19:00 h) and dark (19:00–07:00 h), room temperature was 22 °C ± 2 °C and with free access to food and water. Male rats were kept in two animals per cage, and female rats were kept in three animals per cage. The Animal Care and Use Committee of Kumamoto University approved the protocols for animal experiments (ID: A2021-025), and all methods were performed in accordance with ARRIVE guidelines and relevant and regulations.