Highly selective PPAR β/δ agonist GW0740 and PPAR β/δ antagonist GSK0660 were purchased from MedChem Express (NJ, United States). All other reagents were obtained from the supplier as indicated and were at least analytical grade. The antibodies used and their sources were also indicated below.
Gsk0660
Manufactured by MedChemExpress
Sourced in United States
GSK0660 is a laboratory chemical compound produced by MedChemExpress. It is a highly selective and potent peroxisome proliferator-activated receptor gamma (PPARγ) antagonist. GSK0660 is intended for research use only.
Automatically generated - may contain errors
2 protocols using gsk0660
1
PPAR β/δ Agonist and Antagonist Assay
2
Aortic Senescence Model: Inducing and Treating
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals were euthanized using carbon dioxide. All the operative instruments and body surfaces of animals were sterilized with 75% ethanol. Thoracic aortas were dissected from the surrounding adipose tissues, stored in sterile phosphate buffered solution (PBS), and cut into 1.6-mm length rings. Mouse aortic rings were incubated in an atmosphere of 5% CO
2 at 37°C for 48 h in low-glucose DMEM (Gibco, Carlsbad, USA) containing 10% FBS (Gibco) and 1% antibiotics. Aortic rings were treated with D-gal (10‒100 mM; Sigma-Aldrich, St Louis, USA), GYY4137 (50‒400 μM; Cayman, Ann Arbor, USA), GW501516 (1 μM; MedChemExpress, Monmouth Junction, USA), GSK0660 (1 μM; MedChemExpress) or EMPA (0.5‒10 μM; MedChemExpress) for 48 h. Administration of 50 mM D-gal for 48 h was used to induce aortic senescence.
2 at 37°C for 48 h in low-glucose DMEM (Gibco, Carlsbad, USA) containing 10% FBS (Gibco) and 1% antibiotics. Aortic rings were treated with D-gal (10‒100 mM; Sigma-Aldrich, St Louis, USA), GYY4137 (50‒400 μM; Cayman, Ann Arbor, USA), GW501516 (1 μM; MedChemExpress, Monmouth Junction, USA), GSK0660 (1 μM; MedChemExpress) or EMPA (0.5‒10 μM; MedChemExpress) for 48 h. Administration of 50 mM D-gal for 48 h was used to induce aortic senescence.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!