Spursil c18 column

Aliquots (2 µL) of web extracts of adult and subadult female S. triangulosa were analyzed, and compared, using coupled high-performance liquid chromatography—mass spectrometry (HPLC/MS). The Bruker maXis Impact Quadrupole Time-of-Flight LC/MS system consisted of an Agilent 1200 LC fitted with a Spursil C₁₈ column (30 mm × 3.0 mm, 3 µm; Dikma Technologies, Foothill Ranch, CA, USA) and a Bruker maXis Impact Ultra-High Resolution tandem TOF (UHR-Qq-TOF) mass spectrometer. The LC/MS was operated with positive electrospray ionisation (+ ESI) at a gas temperature of 200 °C and a flow of 9 L/min. The nebuliser was set to 4 bar and the capillary voltage to 4200 V. The column was eluted with a 0.4 mL/min flow of a solvent gradient, starting with 80% water and 20% acetonitrile, and ending with 100% acetonitrile after 4 min. The solvent system contained 0.1% formic acid to improve the peak shape of compounds.

A Bruker maXis Impact Quadrupole Time-of-Flight LC/MS System was used for the analysis. The system consists of an Agilent 1200 HPLC and a Bruker maXis Impact Ultra-High Resolution tandem TOF (UHR-Qq-TOF) mass spectrometer. The Software used was Compass 1.5. For the MS, the ionization mode was Negative Electrospray Ionization (-ESI). Gas Temp: 180°C. Gas Flow: 8 l/min. Nebulizer: 2 bar. Capillary Voltage: 3000 V. Mass Range: 50–1500 Da. Calibrant: Sodium Formate. For the HPLC, a Spursil C18 column with 3 micron particle size, 30 mm length × 3.0 mm diameter (Dikma Technologies) was used. The column temperature was: 30°C. For the solvent gradients, Solvent A was water with 0.1% formic acid; and Solvent B was acetonitrile with 0.1% formic acid.

The bioactive HPLC fractions were analysed on a Bruker maXis Impact Quadrupole Time-of-Flight HPLC/MS System. The system consists of an Agilent 1200 HPLC fitted with a spursil C₁₈ column (30 mm × 3.0 mm, 3 µ; Dikma Technologies, Foothill Ranch, CA, USA) and a Bruker maXis Impact Ultra-High Resolution tandem TOF (UHR-Qq-TOF) mass spectrometer. The LC-MS conditions were as follows: The mass spectrometer was set to positive electrospray ionisation (+ESI) with a gas temperature of 200 °C and a gas flow of 9 L/min. The nebuliser was set to 4 bar and the capillary voltage to 4200 V. The column was eluted with a 0.4-mL/min flow of a solvent gradient, starting with 80% water and 20% acetonitrile and ending with 100% acetonitrile after 4 min. The solvent contained 0.1% formic acid to improve peak shape.