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4000 qtrap mass spectrometer

Manufactured by Agilent Technologies
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The 4000 QTRAP mass spectrometer is a highly sensitive analytical instrument designed for quantitative and qualitative analysis of a wide range of analytes. It utilizes a quadrupole linear ion trap mass analyzer to provide accurate mass measurements and advanced fragmentation capabilities for structural elucidation. The 4000 QTRAP delivers reliable performance and flexibility for a variety of applications in the field of analytical chemistry.

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Lab products found in correlation

Absoluteidq p180 kit, by Biocrates (2 mentions) Agilent 1200 rr hplc system, by Agilent Technologies (2 mentions) Metidq version 5 4 8 db100 boron 2607, by Biocrates (2 mentions) Analyst 1, by AB Sciex (2 mentions) Multiskan mk3 microplate reader, by Thermo Fisher Scientific (2 mentions) High performance liquid chromatography (hplc), by Agilent Technologies (1 mentions)

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Spectrometers (10 citations) 4000 Qtrap (5 citations) 4000 Mass Spectrometer (3 citations)

5 protocols using 4000 qtrap mass spectrometer

1

Metabolomic Profiling of HGSC Ascites

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Targeted metabolomics on cell-free ascites and serum of a subset of 19 HGSC patients was performed using AbsoluteIDQ p180 kits (Biocrates Life Sciences AG, Innsbruck, Austria). The kit allows the identification and (semi-) quantification of metabolites by LC- and flow injection analysis (FIA)-MRM. The samples were analyzed on an AB SCIEX QTrap 4000 mass spectrometer (Framingham, MA, USA) using an Agilent 1200 RR HPLC system (Agilent Technologies, Santa Clara, CA, USA), which were operated with Analyst 1.6.2 (AB SCIEX). The chromatographic column was obtained from Biocrates. The serum samples and additional blanks, calibration standards and quality controls were prepared according to the user manual. All amino acids and biogenic amines were derivatized with phenylisothiocyanate. The experiments were validated using the supplied software (MetIDQ, Version 5-4-8-DB100-Boron-2607, Biocrates). Histamine, methioninesulfoxide, symmetric dimethylarginine, α-aminoadipic acid and trans-4-hydroxyproline did not pass quality controls and therefore, were not considered for data interpretation.
Auer K., Bachmayr-Heyda A., Sukhbaatar N., Aust S., Schmetterer K.G., Meier S.M., Gerner C., Grimm C., Horvat R, & Pils D. (2016). Role of the immune system in the peritoneal tumor spread of high grade serous ovarian cancer. Oncotarget, 7(38), 61336-61354.
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2

Targeted Metabolomic Analysis of Serum

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Targeted metabolomics in serum was performed using AbsoluteIDQ p180 kits (Biocrates Life Sciences AG, Innsbruck, Austria)20 (link). The kit allows the identification and (semi-) quantification of metabolites by liquid chromatography (LC)- and flow injection analysis (FIA)-multiple reaction monitoring (MRM). The samples were analyzed on an AB SCIEX QTrap 4000 mass spectrometer (Framingham, MA, USA) using an Agilent 1200 RR HPLC system (Agilent Technologies, Santa Clara, CA, USA), which were operated with Analyst 1.6.2 (AB SCIEX). The chromatographic column was obtained from Biocrates. The serum samples and additional blanks, calibration standards and quality controls were prepared according to the user manual. All amino acids and biogenic amines were derivatized with phenylisothiocyanate. The experiments were validated using the supplied software (MetIDQ, Version 5-4-8-DB100-Boron-2607, Biocrates). Metabolite concentrations were compared to PD-L1 abundances as described in the Luminex analyses section. A false discovery rate (FDR) cut-off of 5% was used.
Aust S., Felix S., Auer K., Bachmayr-Heyda A., Kenner L., Dekan S., Meier S.M., Gerner C., Grimm C, & Pils D. (2017). Absence of PD-L1 on tumor cells is associated with reduced MHC I expression and PD-L1 expression increases in recurrent serous ovarian cancer. Scientific Reports, 7, 42929.
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3

Quantitative TBBPA Analysis in Sediment

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For the recovery study, a series of TBBPA (0, 500, 1000, 1500, 3000, and 4000 pg) were spiked into the TBBPA free sediment (the dry weight is 1g). The samples were first gently shaken for 10 min in 5 mL water/methanol = 1/1 extraction solution. After centrifugation at 10, 000 × g for 10 min, the supernatant was diluted with assay buffer and subjected directly to the one-step BLEIA based on both NB/Nluc and NB/C4BP/Nluc. The extraction solution was also subjected to LC-MS/MS method following the centrifugation at 3000 × g for 20 min. The analysis by LC-MS/MS was carried out in Agilent HPLC and 4000 Qtrap mass spectrometer along with C18 column (Table S3).
Li Z., Wang Y., Vasylieva N., Wan D., Yin Z., Dong J, & Hammock B.D. (2020). An Ultra-sensitive Bioluminescent Enzyme Immunoassay Based on Nanobody/Nanoluciferase Heptamer Fusion for the Detection of Tetrabromobisphenol A in Sediment. Analytical chemistry, 92(14), 10083-10090.
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4

Microplate Reader and Liquid Chromatography-Mass Spectrometry Protocol

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The absorbance value was measured on a Multiskan MK3 microplate reader (Thermo Labsystems). Luminescence values were measured using a Tecan Infinite M1000-Pro plate reader (Männedorf, Switzerland). Microplates were washed by a Multiskan MK2 microplate washer (Thermo Scientific) and AquaMax Microplate Washer (Bethesda, MA). LC-MS/MS analysis was carried out on an Agilent SL liquid chromatograph system connected to a 4000 Qtrap mass spectrometer (Agilent Technologies, Santa Clara, CA).
Wang F., Li Z.F., Yang Y.Y., Wan D.B., Vasylieva N., Zhang Y.Q., Cai J., Wang H., Shen Y.D., Xu Z.L, & Hammock B.D. (2020). Chemiluminescent Enzyme Immunoassay and Bioluminescent Enzyme Immunoassay for Tenuazonic Acid Mycotoxin by Exploitation of Nanobody and Nanobody-Nanoluciferase Fusion. Analytical chemistry, 92(17), 11935-11942.
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5

Analytical Procedures for Mycotoxin Detection

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ITeA mycotoxin was obtained with a purity of 97.64% (Xiao et al., 2018 (link)). Other mycotoxins including TeA, alternariol monomethyl ether (AME), alternariol (AOH), deoxynivalenol (DON), and zearalenone (ZEN) were purchased from Toronto Research Chemicals (Ontario, Canada) and Aladdin Chemical Technology Co., Ltd. (Shanghai, China). The hapten ITeA-CMO was the carboxymethyloxime derivative of ITeA, which was prepared in our laboratory (Figure S1). The immunogen ITeA-CMO-ConA (ITeA-CMO conjugated with carrier protein concanavalin A (ConA)), and the coating antigen ITeA-CMO-OVA (ITeA-CMO conjugated with ovalbumin (OVA)) were used in this study. The secondary antibody goat anti-HA tag labeled with horseradish peroxidase (anti-HA-HRP) was purchased from Roche Molecular Systems, Inc. (Indianapolis, USA). Other molecular biology reagents were purchased from Gbcbio Technologies Inc. (Guangzhou, China), Takara (Dalian, China), QIAGEN (Dusseldorf, Germany) and New England Biolabs (MA, USA). All other chemicals used in this study were from Sigma (St. Louis, USA). The absorbance value was measured on a Multiskan MK3 microplate reader (Thermo Labsystems, USA). LC-MS/MS analysis was conducted on an Agilent SL liquid chromatograph system with 4000 Qtrap mass spectrometer (Agilent Technologies, Santa Clara, USA).
Wang F., Yang Y.Y., Wan D.B., Li J.D., Liang Y.F., Li Z.F., Shen Y.D., Xu Z.L., Yang J.Y., Wang H., Gettemans J., Hammock B.D, & Sun Y.M. (2022). Nanobodies for Accurate Recognition of Iso-tenuazonic Acid and Development of Sensitive Immunoassay for Contaminant Detection in Foods. Food control, 136, 108835.
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