Anti hck

Manufactured by Santa Cruz Biotechnology

Anti-Hck is a high-quality antibody specifically designed to detect Hck, a member of the Src family of non-receptor protein tyrosine kinases. This antibody can be used for various applications, including Western blotting, immunoprecipitation, and immunocytochemistry, to study the expression and localization of Hck in different cell types and experimental conditions.

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3 protocols using anti hck

Quantitative Western Blotting of Phospho-Src

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Western blotting was performed with anti-phospho-Src (Tyr-416, cell signaling), anti-Lyn, and anti-Hck (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) primary antibodies and with relevant secondary antibodies (Santa Cruz Biotechnology, Inc.) via standard techniques [11 (link)]. Phospho-Src band intensities were determined densitometrically with Image J software, and normalized to Lyn or Hck intensity.

Cheng M., Huang K., Zhou J., Yan D., Tang Y.L., Zhao T.C., Miller R.J., Kishore R., Losordo D.W, & Qin G. (2015). A critical role of Src family kinase in SDF-1/CXCR4-mediated bone-marrow progenitor cell recruitment to the ischemic heart. Journal of molecular and cellular cardiology, 81, 49-53.

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Antibody Validation for Kinase Analysis

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Anti-β-actin and anti-TNF-α antibodies were from Abcam. Anti-Hck and Anti-Yes antibodies were from Santa Cruz Biotechnology. Anti-Lck, anti-Lyn, anti-Fyn, anti-c-Src, and anti-pTyr-416-SFKs antibodies were from Cell Signaling Technology. HRP-conjugated goat anti-mouse and goat anti-rabbit antibodies were from Abcam.

Irtegun S., Pektanc G., Akkurt Z.M., Bozkurt M., Turkcu F.M, & Kalkanli-Tas S. (2016). Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet's Disease. Mediators of Inflammation, 2016, 5414369.

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Src Family Kinase Co-Immunoprecipitation

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For co-immunprecpititation experiments, whole-cell extracts were prepared in lysis buffer (Cell Signaling) with protease inhibitor complex (Roche diagnostics) and subsequently immunoprecipitated by anti-Src Family (phosphor Y416) antibody (CST, #6943 S) which immobilized on protein A/G PLUS-Agarose (Santa Cruz Biotechnology). The immunoprecipitates were resolved by a 10% SDS-PAGE and immunoblotted with antibodies listed below anti-Fyn (CST, #4023 T), anti-Src (CST, #2123 T), anti-Lyn (CST, #2796 T), anti-Lck (Santa Cruz Biotechnology), and anti-Hck (CST, #14643 S) antibodies, respectively.

Du G., Wang J., Zhang T., Ding Q., Jia X., Zhao X., Dong J., Yang X., Lu S., Zhang C., Liu Z., Zeng Z., Safadi R., Qi R., Zhao X., Hong Z, & Lu Y. (2020). Targeting Src family kinase member Fyn by Saracatinib attenuated liver fibrosis in vitro and in vivo. Cell Death & Disease, 11(2), 118.

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