Luciferin luciferase

Manufactured by Chrono-Log

Sourced in United States, United Kingdom

Luciferin-luciferase is a bioluminescent substrate used in analytical applications. It catalyzes the oxidation of luciferin, resulting in the emission of light. This reaction is commonly used for the detection and quantification of ATP and other biological analytes.

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Lab products found in correlation

Adenosine diphosphate (adp), by Chrono-Log (2 mentions) Mepacrine, by Merck Group (1 mentions) Fitc phalloidin, by Merck Group (1 mentions) Hsp27, by Cell Signaling Technology (1 mentions) Total p38, by Cell Signaling Technology (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Phospho hsp27, by Cell Signaling Technology (1 mentions) β actin monoclonal antibody, by Proteintech (1 mentions) Total erk, by Cell Signaling Technology (1 mentions) Thrombin, by Chrono-Log (1 mentions) Chronolog 700 aggregometer, by Chrono-Log (1 mentions) Abciximab, by Eli Lilly (1 mentions) Human α thrombin, by Merck Group (1 mentions) Atp standard, by Chrono-Log (1 mentions) Sch 79797, by Cayman Chemical (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions) Ecl reagent, by GE Healthcare (1 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (1 mentions) Secinh3, by Bio-Techne (1 mentions) Gapdh antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Luciferin/luciferase reagent (13 citations) Chrono‐Lume luciferin‐luciferase reagent (2 citations) Luciferin/luciferase kit (2 citations)

4 protocols using luciferin luciferase

Platelet Activation Signaling Pathways

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Collagen, thrombin, ADP and luciferin/luciferase were provided by the Chrono-Log Corporation. FITC-phalloidin, H₂O₂ and mepacrine were purchased from Sigma-Aldrich; Merck KGaA. Antibodies against phospho-ERK1/2 (catalog no. 4370S), phospho-p38 (catalog no. 4511S), phospho-HSP27 (catalog no. 2401S), total-p38 (catalog no. 8690S), total-ERK (catalog no. 4695S) and HSP27 (catalog no. 95357S) were purchased from Cell Signaling Technology Inc. β-actin monoclonal antibody (catalog no. 66009-1-Ig) was obtained from ProteinTech Group, Inc. The antibody for integrin β3 (D-11) (catalog no. sc-365679) was obtained from Santa Cruz Biotechnology Inc. PAC-1 antibodies (catalog no. MA5-28564) were from Invitrogen; Thermo Fisher Scientific, Inc., and CD62P antibodies (catalog no. 555524) were from BD Biosciences.

Ruan Y., Ding Y., Li X., Zhang C., Wang M., Liu M., Wang L., Xing J., Hu L., Zhao X., Ding Z., Dong J, & Liu Y. (2022). Saccharides from Arctium lappa L. root reduce platelet activation and thrombus formation in a laser injury thrombosis mouse model. Experimental and Therapeutic Medicine, 23(5), 344.

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Simultaneous Platelet Aggregation and ATP Secretion

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Simultaneous monitoring of platelet aggregation and ATP secretion was performed at 37 °C with constant stirring (1000 rpm) in a Chronolog 700 aggregometer (Chronolog, Havertown, PA, USA). For all experiments involving pharmacological inhibitors, platelets (2 × 10⁸ mL^− 1) were pre-treated with vehicle control or inhibitors for 10 min. Also, for experiments involving functional blockade of platelet CXCR7, platelets were pre-incubated for 10 min with 10 μg/mL^− 1 IV.3 prior to a 10 min incubation with 10 μg/mL^− 1 anti-CXCR7 (11G8) or IgG₁ control to prevent non-specific platelet activation via the FcγRIIA receptor. Before agonist stimulation, 5 μL of luciferin-luciferase (Chronolog) was added. Post aggregation, 1 nM ATP standard was added as a reference value for quantification of secreted ATP. Data analysis was performed with Aggro/Link5 software (Chronolog).

Walsh T.G., Harper M.T, & Poole A.W. (2015). SDF-1α is a novel autocrine activator of platelets operating through its receptor CXCR4. Cellular Signalling, 27(1), 37-46.

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Purification and Characterization of HuPK and rBbKI

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HuPK was purified as previously described [14 (link)] and the specific activity was found to be 80 U/mg. Recombinant Bauhinia bauhinioides kallikrein inhibitor (rBbKI) was obtained as described [15 (link)]. ADP, ATP-standard, and luciferin-luciferase were from Chrono-Log (Chester, PA, USA). Human α-thrombin (200 NIH units/mg) was from Sigma (St. Louis, MO, USA). The PAR-1 antagonist SCH 79797 was from Cayman Chemical (Ann Arbor, MI, USA); abciximab was from Eli Lilly and Company (Indianapolis, IN, USA), while Fluo-4-AM was from Thermo Fisher Scientific Corporation (Waltham, MA, USA). TLCK was purchased from Merck KGaA /Calbiochem (Darmstadt, Germany).

Ottaiano T.F., Andrade S.S., de Oliveira C., Silva M.C., Buri M.V., Juliano M.A., Girão M.J., Sampaio M.U., Schmaier A.H., Wlodawer A., Maffei F.H, & Oliva M.L. (2017). Plasma Kallikrein enhances platelet aggregation response by subthreshold doses of ADP. Biochimie, 135, 72-81.

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Antibody and Reagent Identification for Protein Analysis

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The phospho-(Ser) PKC substrate (pSer PKC substrate) antibody was from Cell Signaling Technology (Danvers, MA, USA). The cytohesin-2 western blot antibody, ARF6 antibody and GAPDH antibody were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The cytohesin-2 immunoprecipitation antibody was from Thermo Scientific (Loughborough, Leicestershire, UK). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit, anti-mouse and anti-goat secondary antibodies were from Jackson ImmunoResearch Laboratories (Newmarket, UK). The PKC inhibitor bisindolylmaleimide (BIM) IX (Ro 31-8220) and the inactive analogue BIM V were from Calbiochem (Nottingham, UK). The PKC inhibitors BIM I (GF 109203X), Go 6983 and ruboxistaurin (LY 333531) and the cytohesin-2 inhibitor SecinH3 were from Tocris (Bristol, UK). The GST-GGA3 construct was a generous gift from Professor Sidney Whiteheart (University of Kentucky, USA). Cross-linked collagen-related peptide (CRP) was synthesized by Professor Richard Farndale (University of Cambridge, UK). PE-P-selectin and FITC-PAC1 antibodies were from Emfret Analytics (Eibelstadt, Germany). Luciferin-luciferase was from Chronolog (Labmedics, Stockport, UK). NuPAGE LDS sample buffer was from Invitrogen (Carlsbad, CA, USA). ECL reagent was from GE Healthcare (Amersham, UK). All other reagents were from Sigma-Aldrich (Poole, UK).

van den Bosch M.T., Poole A.W, & Hers I. (2014). Cytohesin-2 phosphorylation by protein kinase C relieves the constitutive suppression of platelet dense granule secretion by ADP-ribosylation factor 6. Journal of Thrombosis and Haemostasis, 12(5), 726-735.

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