Uplfln40xo

Cardiomyocyte sarcomere dynamics in the posterior half of the second heart chamber of 30-day-old GFP α-actinin [CC01961] animals (Buszczak et al., 2007 (link)) were imaged using 19 mW per mm² blue excitation light, delivered through a 40x/1.3 NA objective (Olympus UPLFLN40XO), with the excitation pattern again restricted to the field of view of the acquired image. Images were captured at 60 frames per second but with otherwise identical camera settings as above. The heart wall detection algorithm was adapted to trace single sarcomeres in YT kymographs of paired left and right cardiomyocytes. The data were subsequently analyzed by calculating the amplitude of coherence between the two sarcomeres: ${\displaystyle Coherence=-\frac{\mathrm{\partial }Positio{n}_{Left}}{\mathrm{\partial }Time}\cdot \frac{\mathrm{\partial }Positio{n}_{Right}}{\mathrm{\partial }Time}}$