The CSF analyses of T-tau (INNOTEST®hTau Ag) and phospho-tau181 (P-tau181) (INNOTEST® PHOSPHO-TAU (181P)) were performed using Innogenetics enzyme-linked immunosorbent assays (ELISA, Innogenetics, Ghent, Belgium). The NFL ELISA (UmanDiagnostics NF-light®, Umeå, Sweden) was performed according to a previously established protocol,24 (link) with minor modifications. The analysis of MBP was performed by an ELISA (Active® MBP, Diagnostic Systems Laboratories, Webster, TX, USA) purchased from Diagnostic Systems Laboratories. CSF levels of YKL-40 and sCD14 were analyzed by ELISAs (Quantikine® ELISA, Human Chitinase 3-like 1 Immunoassay and Human sCD14 Immunoassay, both from R&D Systems Inc, Abingdon, UK). MCP-1 was measured by an ultra-sensitive ELISA (Human MCP-1 Ultra-Sensitive kit, Meso Scale Discovery, Rockville, MD, USA). All the commercial assays were analyzed according to the instructions given by the manufacturers. GFAP was measured by a previously described ELISA procedure.25 (link) The coefficients of variation for all biochemical analyses were below 10%.
Human mcp 1 ultra sensitive kit
Manufactured by Mesoscale
Sourced in United States
The Human MCP-1 Ultra-Sensitive kit is a laboratory tool designed to detect and quantify the human monocyte chemoattractant protein-1 (MCP-1) in biological samples. This kit utilizes a highly sensitive enzyme-linked immunosorbent assay (ELISA) technique to measure MCP-1 levels with high accuracy and precision.
Automatically generated - may contain errors
2 protocols using human mcp 1 ultra sensitive kit
1
Comprehensive CSF Biomarker Analysis
2
Multiplex Cytokine and Biomarker Assays
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IL-8 was analyzed together with IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, TNF-α, and IFN-γ using the MSD 96-well multi-array and multi-spot human cytokine assay (Human Cytokine Assay Ultra-Sensitive kit, Meso Scale Discovery). MCP-1 concentration was measured using a commercial electrochemiluminescence enzymelinked immunosorbant assay (ELISA; Human MCP-1 Ultra-Sensitive Kit, Meso Scale Discovery). YKL-40 concentration was measured using a commercial colorimetric ELISA (Human chitinase-3 quantikine ELISA kit, R&D systems Inc.). NF-L concentration was measured with a commercial ELISA assay (NFLight, UmanDiagnostis AB, Umeå, Sweden).
All analyses were performed according to the manufacturers' instructions at the Clinical Neurochemistry Laboratory in Mölndal, Sweden. Intra-assay coefficients of variation were below 10% for all assays. The staff performing the analyses was blinded to all phenotype information.
All analyses were performed according to the manufacturers' instructions at the Clinical Neurochemistry Laboratory in Mölndal, Sweden. Intra-assay coefficients of variation were below 10% for all assays. The staff performing the analyses was blinded to all phenotype information.
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