6 well culture plate

Manufactured by BD

Sourced in United States

The 6-well culture plates are a type of laboratory equipment used for cell culture applications. They provide a standardized, multi-well format for the growth and maintenance of cells in a controlled environment.

Automatically generated - may contain errors

Lab products found in correlation

Dmem high glucose with pyruvate, by Thermo Fisher Scientific (2 mentions) 96 well black clear bottom culture plate, by Corning (2 mentions) 10 kda cut off membrane, by Merck Group (2 mentions) Dmem lg, by Thermo Fisher Scientific (1 mentions) Mem non essential amino acid, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) Ascorbate 2 phosphate, by Merck Group (1 mentions) β glycerophosphate, by Merck Group (1 mentions) Pha 665752, by Merck Group (1 mentions) Tgf β1, by Sino Biological (1 mentions) Methyl 3h thymidine, by PerkinElmer (1 mentions) Ml141, by Selleck Chemicals (1 mentions) Nsc23766, by Selleck Chemicals (1 mentions) Cell culture insert, by BD (1 mentions) Dulbecco s modified eagle s medium, by Merck Group (1 mentions) Dispase, by Roche (1 mentions)

Spelling variants of this product

6-well plates (299 citations) Tissue culture 6-well plates (4 citations) Cell Culture Inserts for 6-well plates (2 citations) Tissue-culture treated adherent 6-well plates (2 citations)

32 protocols using 6 well culture plate

Rat iPSC Osteogenic Induction Protocol

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The iPSCs were reprogrammed from Sprague Dawley rat fibroblasts by transduction of retroviral vectors encoding four transcription factors (Oct-4/Sox2/Klf4/c-Myc; OSKM; Supplementary Information)^{43 (link)}. In brief, undifferentiated iPSCs were routinely cultured and expanded on mitotically inactivated REFs (50,000 cells/cm²) in 6-well culture plates (BD, Franklin Lakes, New Jersey, United States) in the presence of 0.3% leukemia inhibitory factor in an iPSCs medium that consisted of DMEM (Sigma-Aldrich, St. Louis, Missouri, United States) supplemented with 15% FBS (Invitrogen, Waltham, Massachusetts, United States), 100 mM MEM nonessential amino acids (Sigma-Aldrich, St. Louis, Missouri, United States), 0.55 mM 2-mercaptoethanol (Gibco, Waltham, Massachusetts, United States), and antibiotics (Invitrogen, Waltham, Massachusetts, United States). For osteogenic induction, iPSCs were cultured in DMEM-LG (Invitrogen, Waltham, Massachusetts, United States) supplemented with 15% FBS, 50 μg/m L ascorbate-2-phosphate, 10 nmol/L dexamethasone, and 10 mmol/L β-glycerophosphate (Sigma, St. Louis, MO) for 2 weeks^{44 (link),45 (link)}.

Chien K.H., Chang Y.L., Wang M.L., Chuang J.H., Yang Y.C., Tai M.C., Wang C.Y., Liu Y.Y., Li H.Y., Chen J.T., Kao S.Y., Chen H.L, & Lo W.L. (2018). Promoting Induced Pluripotent Stem Cell-driven Biomineralization and Periodontal Regeneration in Rats with Maxillary-Molar Defects using Injectable BMP-6 Hydrogel. Scientific Reports, 8, 114.

+ Open protocol

+ Expand

Optimizing LPS Concentrations for In Vitro Parasite-Bacteria Interaction

Check if the same lab product or an alternative is used in the 5 most similar protocols

To choose the optimal LPS concentrations for an in vitro model of parasite–bacteria interaction, the preliminary culture of A. simplex s.s. L3 larvae was conducted under anerobic conditions (37 °C, 5% CO₂) in the presence of LPS isolated from E. coli (Sigma–Aldrich; L8274-10MG) at different concentrations: 0.05, 0.1, 0.15, 0.2, 0.4, 0.6, 0.8, 1, and 2 μg/ml for 12 h. The procedure of in vitro culture was carried out as described previously by Iglesias et al. (38 (link)) using the reagents as described before (36 , 39 ). Three A. simplex s.s. L3 larvae were placed in each well of the 6-well culture plates (BD Biosciences) (27 in total). The parasites without LPS were cultured as a control (three larvae × three wells; nine in total).

Mierzejewski K., Stryiński R., Łopieńska-Biernat E., Mateos J., Bogacka I, & Carrera M. (2021). A Complex Proteomic Response of the Parasitic Nematode Anisakis simplex s.s. to Escherichia coliLipopolysaccharide. Molecular & Cellular Proteomics : MCP, 20, 100166.

+ Open protocol

+ Expand

TGF-β1 and PHA665752 Modulate Fibronectin Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

MRC-5 cells were plated at a density of 2 × 10⁵ cells/well in 6-well culture plates (BD Biosciences) and cells were treated with or without indicated concentrations of PHA665752 (Sigma-Aldrich) and 2.5 ng/mL TGF-β1 (Sino Biological) for 24 hours. After removing the medium, hypoxia-pretreated MSCs in transwells were co-cultured with the PHA665752-treated MRC-5 cells for 24 hours. The MRC-5 cells were harvested for detection of fibronectin mRNA expression level by quantitative real-time RT-PCR.

Lan Y.W., Choo K.B., Chen C.M., Hung T.H., Chen Y.B., Hsieh C.H., Kuo H.P, & Chong K.Y. (2015). Hypoxia-preconditioned mesenchymal stem cells attenuate bleomycin-induced pulmonary fibrosis. Stem Cell Research & Therapy, 6(1), 97.

+ Open protocol

+ Expand

Thymidine Incorporation Assay for β-TC-6 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

β-TC-6 cells (3 × 10⁵ cells/well) were seeded in 6-well culture plates (BD Bioscience) in DMEM medium supplemented with 20% FBS, penicillin and streptomycin. The following day the β-TC-6 cell medium was removed, and the HSC conditioned medium, supplemented with glucose and FBS, was added to the β-TC-6 cells. Each well received 0.5 µCi of Thymidine [Methyl-³H] (Perkin Elmer, San Jose, CA) and was incubated for 8, 24 and 30 hours at 37°C. After incubation the medium was removed and cells were washed with phosphate-buffered saline, 0.5 mL of 10% trichloroacetic acid was then added to each well for 1 hour to precipitate the DNA. Unbound thymidine was removed by a gentle wash with 4°C phosphate-buffered saline. The DNA was then dissolved in 500 µL of 0.1% Triton X₁₀₀ in 10% sodium hydroxide. The final lysates were transferred to a PET flexible microplate (Perkin Elmer, San Jose, CA) containing 600 µL of scintillation fluid. Measurements were obtained using the 1450 LSC Microbeta TriLux Counter from Perkin Elmer.

Bayan J.A., Peng Z., Zeng N., He L., Chen J, & Stiles B.L. (2015). Crosstalk between Activated Myofibroblasts and β-cells in Injured Mouse Pancreas. Pancreas, 44(7), 1111-1120.

+ Open protocol

+ Expand

Co-culture of Pancreatic Cancer Cells and PSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pancreatic cancer cells were seeded at a density of 2×10⁵ cells/well into 6-well culture plates (BD Biosciences, Franklin Lakes, NJ, USA). Human PSCs were seeded at 4×10⁵ cells/culture insert into culture inserts with 1.0 µm pores (BD Biosciences). The subsequent day, the culture inserts seeded with PSCs were placed into the 6-well plates containing pancreatic cancer cells, and incubation was continued for ≤3 days at 37°C.

Liu S.L., Cao S.G., Li Y., Sun B., Chen D., Wang D.S, & Zhou Y.B. (2018). Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion. Oncology Letters, 17(2), 2057-2062.

+ Open protocol

+ Expand

Evaluating Pancreatic Cancer Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols

The migratory ability of pancreatic cancer cells was evaluated using a wound-healing assay. Each pancreatic cancer cell line was cultured at 37 °C and 5% CO₂ in 6-well culture plates (BD Biosciences, San Jose, CA, USA) to 80% confluence. The suspended cells were removed with three washes of phosphate-buffered saline (PBS). A sterilised pipette tip was then used to create a wound (scratch) in the confluent layer. Next, DFX (0, 10, 50, or 100 μM), the Rac-1 inhibitor NSC 23766 (0, 50, 100, 200 μM; Selleck, Houston, TX, USA), or the Cdc42 inhibitor ML141 (0, 10, 20, 40 μM; Selleck) were added to each well, and the cells were incubated for 24 h. Afterwards, the wound width was measured; the ratio of pre- and post-culture wound widths was used as an index of cell migration, and comparisons were made with the control group.

Amano S., Kaino S., Shinoda S., Harima H., Matsumoto T., Fujisawa K., Takami T., Yamamoto N., Yamasaki T, & Sakaida I. (2020). Invasion inhibition in pancreatic cancer using the oral iron chelating agent deferasirox. BMC Cancer, 20, 681.

+ Open protocol

+ Expand

Conditioned Medium Effects on β-TC-6 Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Conditioned medium from PSCs and primary rat HSCs were collected from 3 day cell cultures. β-TC-6 cells (3 × 10⁵ cells/well) were seeded in 6-well culture plates (BD Bioscience) in DMEM medium supplemented with 20% FBS, penicillin and streptomycin. The following day, the β-TC-6 cell medium was removed and washed with PBS. Then, the conditioned medium, supplemented with glucose and FBS, was added to the β-TC-6 cells. β-TC-6 cells were typsinized and counted after 3–6 days.

Bayan J.A., Peng Z., Zeng N., He L., Chen J, & Stiles B.L. (2015). Crosstalk between Activated Myofibroblasts and β-cells in Injured Mouse Pancreas. Pancreas, 44(7), 1111-1120.

+ Open protocol

+ Expand

IL-6 Regulation in MSCs by VCA Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze the activities of IL-6 in MSCs following VCA treatment, PD-MSCs and BM-MSCs were seeded in 6-well culture plates (BD Biosciences) at 1.0 × 10⁴ cells per well. After 24 h, the cells were treated with various concentrations of VCA (0, 1, 5, 10 and 100 pg/mL) for 48 h. The culture media were collected and preserved at −80 °C, and the activities of IL-6 were measured using human-specific ELISA kits (BD Biosciences) according to the manufacturer’s instructions. All experiments were performed in two separate replicates.

Kim G.D., Choi J.H., Lim S.M., Jun J.H., Moon J.W, & Kim G.J. (2019). Alterations in IL-6/STAT3 Signaling by Korean Mistletoe Lectin Regulate the Self-Renewal Activity of Placenta-Derived Mesenchymal Stem Cells. Nutrients, 11(11), 2604.

+ Open protocol

+ Expand

Bioengineered Tooth Germ Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Molar tooth germs were dissected from the mandibles of ED14.5 C57BL/6 and C57Bl/6-Tg (CAG-EGFP) mice20 (link)33 (link) (Fig. 1a). The isolated tooth germs were incubated in 1.2 U/ml dispase (Roche) for 10 min at room temperature. The epithelium and mesenchymal tissues were separated using a fine needle. The mesenchymal tissues of the C57BL/6 mice were placed into a 30-ml gel drop of Cellmatrix type I-A (Nitta gelatin), and the epithelial tissues of the C57BL/6-Tg (CAG-EGFP) mice were then placed on the mesenchymal tissues. The bioengineered tooth germs were placed on a cell culture insert (0.4-mm-diameter pore, BD) and incubated at 37°C in a humidified atmosphere at 5% CO₂. The reconstituted explants were cultured for 3 days on cell culture inserts in 6-well culture plates (BD) in Dulbecco's modified Eagle's medium (Sigma) supplemented with 10% fetal bovine serum (FBS).

Yajima-Himuro S., Oshima M., Yamamoto G., Ogawa M., Furuya M., Tanaka J., Nishii K., Mishima K., Tachikawa T., Tsuji T, & Yamamoto M. (2014). The junctional epithelium originates from the odontogenic epithelium of an erupted tooth. Scientific Reports, 4, 4867.

+ Open protocol

+ Expand

Hypoxia-Primed Mesenchymal Stem Cells Enhance Fibronectin Expression in Lung Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

MSCs were plated at a density of 1 × 10⁵ cells/well and MRC-5 cells were plated at a density of 2 × 10⁵ cells/well in transwells (BD Biosciences) and 6-well culture plates (BD Biosciences), respectively, and the cells were cultured overnight. MSCs were then treated with the indicated oxygen concentrations in hypoxic treatment for 24 hours. MRC-5 cells were treated with or without 2.5 ng/mL transforming growth factor (TGF)-β1 (Sino Biological Inc., Beijing, China) for 24 hours. After removing the medium, hypoxia-pretreated MSCs in transwells were co-cultured with the MRC-5 cells for 24 hours. The MRC-5 cells were harvested for detection of fibronectin mRNA expression level by quantitative real-time RT-PCR.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!