Ez1 virus mini kits v2

DNA was prepared from each sample using EZ1 Virus Mini Kits v2.0 (Qiagen, Hilden, Germany) according to the instructions from the manufacturer. Quantitative real-time polymerase chain reaction (PCR) assay was used to measure the PVL of HTLV-1 in cells, as described previously (Matsuda et al., 2005 (link); Fukui et al., 2017 (link)). PVL was quantified using the HTLV-1 Tax primer (forward, 5’-CCCACTTCC CAGGGTTTGGA-3′; reverse, 5’-GGCCAGTAGGGCG TGA-3′) and probe (5’-FAM-CCAGTCTACGTGTTTGGA GACTGTGTACA-TAMRA-3′). Glyceraldehyde-3-phosphate dehydrogenase was used as the internal control.

MT2 or TL-Om1 cells were cultured in RPMI1640 containing 10% FBS and 1% P/S with 0 or 10 μg/ml of adalimumab. EZ1 Virus Mini Kits v2.0 (Qiagen, Hilden, Germany) was used to prepare the DNA from each sample. PVL of HTLV-1 in cells was measured using quantitative real-time PCR assay as described previously (Matsuda et al., 2005 (link); Fukui et al., 2017 (link); Uchida et al., 2019 (link)). PVL was quantified using the HTLV-1 Tax primer (forward, 5'-CCCACTTCC CAGGGTTTGGA-3'; reverse, 5'-GGCCAGTAGGGCG TGA-3') and probe (5'-FAM-CCAGTCTACGTGTTTGGA GACTGTGTACA-TAMRA-3'). Glyceraldehyde-3-phosphate dehydrogenase was used as the internal control.