Iraffinity

Manufactured by Shimadzu

Sourced in Japan, United States

The IRAffinity is a Fourier Transform Infrared (FTIR) Spectrophotometer manufactured by Shimadzu. It is designed for high-performance infrared spectroscopy analysis. The core function of the IRAffinity is to measure the absorption and transmission of infrared light by samples, providing information about their molecular structure and composition.

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Lab products found in correlation

Gladiatr, by PIKE Technologies (3 mentions) Nicolet is50, by Thermo Fisher Scientific (2 mentions) Maldi tof ms, by Bruker (1 mentions) Fe sem s 4800, by Hitachi (1 mentions) Ez test, by Shimadzu (1 mentions) 4 hydroxycoumarin, by Merck Group (1 mentions) Drx 400 spectrometer, by Bruker (1 mentions) Methylimidazole, by Merck Group (1 mentions) 2 aminopyridine, by Merck Group (1 mentions) Dv420a, by Oxford Instruments (1 mentions) Uv 3101pc, by Shimadzu (1 mentions) J 815 spectrometer, by Jasco (1 mentions) Lc 20ar prominence, by Shimadzu (1 mentions) P 1010 polarimeter, by Jasco (1 mentions) Atr ftir, by Shimadzu (1 mentions) Mira3 model, by TESCAN (1 mentions) Nanotrac wave 2, by Microtrac (1 mentions) Ultima 4, by Rigaku (1 mentions) Aaf 1100, by Carbolite (1 mentions) Fourier transform infrared spectroscopy, by Shimadzu (1 mentions)

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22 protocols using iraffinity

Comprehensive Structural Characterization of SCPs1

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FTIR, XRD and MALDI-TOF-MS were used to characterize the structure and determine the molecular weight of the optimal component, SCPs1.
The method used was a modified version of that followed by Wang et al. [21 (link)]. SCPs1 sample powder (1 mg) was mixed with KBr to prepare one pellets. The sample was placed in a Fourier-transform infrared spectrometer (IR Affinity, Shimadzu Inc., Tokyo, Japan,) with a wave number range of 400–4000 cm⁻¹, resolution of 4 cm⁻¹ and scan number of 32 to determine the secondary structure of SCPs1.
XRD analysis of the sample crystal structure was performed using an XRD instrument (D8 ADVANCE, Bruker Inc., Ettlingen, Germany) with Cu as the cathode, a voltage of 40 kV, a current of 200 mA, a scanning speed of 0.02°/min and a scanning range of 5–90° [22 (link)].
MALDI-TOF-MS (Bruker Daltonics Inc., Ettlingen, Germany) was used to determine the relative molecular weight distribution of the sample based on a modified protocol [23 (link)]. The sample was mixed with CHCA matrix in equal proportions, spotted onto a MALDI plate and air-dried; the molecular weight distribution range was measured in positive ion reflection mode, with a lens voltage of 8.02 kV and a resolution of 40,000.

Zu X.Y., Li M.J., Xiong G.Q., Cai J., Liao T, & Li H.L. (2023). Silver Carp (Hypophthalmichthys molitrix) Scales Collagen Peptides (SCPs): Preparation, Whitening Activity Screening and Characterization. Foods, 12(7), 1552.

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FTIR Analysis of Starch Functional Groups

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The identification of the functional groups will be carried out by Fourier transform infrared (FTIR) spectroscopy in a medium spectrum range. FTIR spectra were recorded on an FTIR spectrometer (Shimadzu model IR-Affinity, Kyoto Japan) at room temperature. Briefly, small samples of starch were mixed in a starch: KBr ratio of 1:100 (w/w), dried, and IR spectra were recorded in the wavelength region between 400–4000 cm⁻¹ with a resolution of 4 cm⁻¹. Intensity measurements were made on the spectra by recording the heights of the absorbance bands from the baseline.

Mieles-Gómez L., Quintana S.E, & García-Zapateiro L.A. (2023). Ultrasound-Assisted Extraction of Mango (Mangifera indica) Kernel Starch: Chemical, Techno-Functional, and Pasting Properties. Gels, 9(2), 136.

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Membrane Characterization and Grafting Quantification

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All membrane samples were cleaned with DI water and dried in a vacuum oven (12.5 L, VWR International, Radnor, PA, USA) overnight prior to characterization. Static contact angle measurements were carried out at different ionic strengths of solution. Quintuplicate measurements were made to determine the standard deviation.
FTIR (IRAffinity, Shimadzu, MD, USA) was performed to determine characteristic functional groups of the modified membranes. XPS (Thermo Fisher Scientific Inc., Waltham, MA, USA) was used for analyzing the chemical compositions of the membranes before and after modification. SEM (FESEM S-4800, Hitachi Co., Tokyo, Japan) was used to characterize the surface fiber structures of both modified and unmodified membranes.
Grafting degree (GD) was calculated to quantitatively determine the amount of PVCL grafted. The membranes before and after modification were dried in a vacuum oven at 40 °C and the weight of the samples was thereafter measured. GD was calculated based on the following equation:

GD (%) = \frac{W_{modified} - W_{unmodified}}{W_{unmodified}} \times 100 %

Chen S.T., Wickramasinghe S.R, & Qian X. (2022). Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification. Membranes, 12(7), 714.

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Characterization and Purification of Organic Compounds

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Unless otherwise noted, commercially
available solvents and reagents were used. The reaction was carried
out in a 30 mL reaction tube. To perform thin layer chromatography
(TLC), 0.25 mm silica gel-coated plates were used. ¹H NMR
(400 MHz), ¹³C NMR (100 MHz), and DEPT-135 NMR spectra
were recorded on a Bruker DRX400 spectrometer using trimethylsilane
(TMS) as an internal standard. The peak multiplicities are assigned
as s (singlet), d (doublet), dd (doublet of doublet), and m (multiplet).
FT-IR spectra were recorded for some selected compounds using an IRAffinity
(SHIMADZU) and Nicolet iS50 (Thermo Scientific) spectrometer on a
KBr disc or ATR mode. Some selected compounds and all new compounds
were analyzed by HRMS analysis. All chemical shifts (δ) are
reported in ppm from TMS and d-solvent as the internal
standard reference. The coupling constants (J) are
reported in Hz. 100–200 mesh silica gel was used for column
chromatography and separated by using the mixture of ethyl acetate
and hexane solvent. The column elution is mentioned in percentage
concerning the volume ratio of ethyl acetate/hexane ratios. All of
the spectroscopic data for the products matched the reported literature
listed in the reference in every way.

Kumar S., Padala K, & Maiti B. (2023). H2O2-Mediated Synthesis of a Quinazolin-4(3H)-one Scaffold: A Sustainable Approach. ACS Omega, 8(36), 33058-33068.

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Infrared Spectral Analysis of Films

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Infrared spectra of films were recorded in the range of 4000–500 cm⁻¹ using a Fourier-Transform Infrared Analyser (FTIR) Shimadzu IR-Affinity (Shimadzu Co., Kyoto, Japan) equipped with an attenuated total reflectance diamond module (GladiATR, Pike Technologies, Madison, WI, USA). The spectra were obtained as an average of 48 scans with a resolution of 4.0 cm⁻¹ and Happ–Genzel apodisation. A blank spectrum was obtained before each test to compensate for the effect of humidity and the presence of carbon dioxide in the air through spectra subtraction. The spectra were obtained in triplicate.

Lago A., Delgado J.F., Rezzani G.D., Cottet C., Ramírez Tapias Y.A., Peltzer M.A, & Salvay A.G. (2023). Multi-Component Biodegradable Materials Based on Water Kefir Grains and Yeast Biomasses: Effect of the Mixing Ratio on the Properties of the Films. Polymers, 15(12), 2594.

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Infrared Spectroscopy Analysis Protocol

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The tests were carried out using Shimadzu IR-Affinity infrared spectrophotometer (Shimadzu Co., Beijing, China) equipped with a diamond-tipped ATR module (GladiATR, Pike Technologies, Fitchburg, WI, USA). The spectra were obtained in duplicate in the range of 4000 to 500 cm⁻¹, 48 scans and a resolution of 4 cm⁻¹. A blank spectrum in air was obtained before each test to compensate the humidity effect and the presence of carbon dioxide.

Rezzani G.D., Choque E., Salvay A.G., Mathieu F, & Peltzer M.A. (2022). New Antioxidant Active Packaging Films Based on Yeast Cell Wall and Naphtho-γ-Pyrone Extract. Polymers, 14(10), 2066.

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Optimizing Transferosome Formulations

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To provide the optimum interaction, the drug and excipient ratio in the formulation of transferosomes was studied at a 1:1 proportion. Additionally, the interaction between the drug and excipient was verified by FTIR (Shimadzu IR Affinity, Kyoto, Japan) in the scanning range of 4000–400 cm⁻¹ using the KBr technique [25 (link)].

Tamilarasan N., Yasmin B.M., Anitha P., Umme H., Cheng W.H., Mohan S., Ramkanth S, & Janakiraman A.K. (2022). Box–Behnken Design: Optimization of Proanthocyanidin-Loaded Transferosomes as an Effective Therapeutic Approach for Osteoarthritis. Nanomaterials, 12(17), 2954.

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Characterization of Plasticized PLA/PBAT Films

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The light microscope was used for onsite examination of sample morphology.
Chemical properties of samples were examined using Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) Shimadzu IRaffinity equipment, Kyoto, Japan, by scanning samples from 4000 to 400 cm⁻¹, with a resolution of 4 cm⁻¹, and following changes in obtained spectra.
Thermal properties were determined using the differential scanning calorimetry (DSC) method on TA Instruments Q20 equipment (New Castle, DE, USA) by heating samples with a heat flow of 10 °C/min in one cycle. The samples were sampled from the casted films and from the coated film made of plasticized PLA/PBAT including all the film thickness.
Shimadzu EZ-Test, Kyoto, Japan instrument was used for mechanical properties assessment. Samples were cut in a rectangular shape of dimensions 1 cm × 4 cm, 0.3 mm thickness and tested with a clamp speed of 10 mm/min.

Miletić A., Ristić I., Coltelli M.B, & Pilić B. (2020). Modification of PLA-Based Films by Grafting or Coating. Journal of Functional Biomaterials, 11(2), 30.

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ATR-FTIR Membrane Surface Analysis

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ATR-FTIR spectroscopy provides qualitative information about functional groups at the top, approximately 2 µm of the membrane. Data were collected using an IR Affinity instrument (Shimadzu, Columbia, MD, USA) with a horizontal ZnSe accessary. ATR-FTIR spectra were averaged over 100 scans covering a range of 1150–3650 cm⁻¹. Prior to analysis, the membranes were dried overnight in a vacuum oven at 40 °C.

Sengupta A., Vu A., Qian X, & Wickramasinghe S.R. (2021). Remote Performance Modulation of Ultrafiltration Membranes by Magnetically and Thermally Responsive Polymer Chains. Membranes, 11(5), 340.

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Synthesis and Characterization of Coumarin Derivatives

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4-Hydroxy coumarin,
substituted aromatic aldehydes, 2-amino pyridine, methylimidazole,
2-chloro acetic acid, and sodium tetrafluoroborate were obtained from
Sigma-Aldrich, and organic solvents were procured from commercial
suppliers and used without any further purification. Analytical thin-layer
chromatography (TLC) was carried out using 0.25 mm silica gel-coated
Kieselgel 60 F254 plates. ¹H NMR (400 MHz), ¹³C NMR (100 MHz), ¹⁹F NMR (100 MHz), and ¹⁰B
NMR (128 MHz) spectra were measured on a Bruker DRX400 spectrometer.
Chemical shifts and coupling constants are specified in parts per
million (ppm) and Hertz (Hz), respectively, using tetra-methyl silane
as an internal standard and the solvent resonance at (DMSO-d₆: ¹H NMR 400 MHz and ¹³C NMR 100 MHz): δ
2.49 and 39.7 ppm. The peak multiplicities are allocated as s (singlet),
d (doublet), dd (doublet of doublet), dt (doublet of triplet), and
m (multiplet). FT-IR spectra were recorded using a IRAffinity (SHIMADZU)
and Nicolet iS50 (Thermo Scientific) spectrometer on a KBr disc. UV–Vis
absorption spectra were recorded on a JASCO V-670 spectrophotometer
at room temperature in acetonitrile. A Hitachi F-7000 FL spectrophotometer
was utilized to record emission fluorescence spectra by excitation
at their respective absorption maxima.

Pasuparthy S.D, & Maiti B. (2022). [CMMIM][BF4–] Ionic Liquid-Catalyzed Facile, One-Pot Synthesis of Chromeno[4,3-d]pyrido[1,2-a]pyrimidin-6-ones: Evaluation of Their Photophysical Properties and Theoretical Calculations. ACS Omega, 7(43), 39147-39158.

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