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Ab26120

Manufactured by Abcam
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Ab26120 is a product offered by Abcam. It is a laboratory equipment item. No further details are available about its core function or intended use while maintaining an unbiased and factual approach.

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Lab products found in correlation

Ab96926, by Abcam (2 mentions) Epcam g8, by BioLegend (2 mentions) Cd103 m290, by BD (2 mentions) Cd11c hl3, by BD (2 mentions) B220 ra3 6b2, by Thermo Fisher Scientific (2 mentions) Avidin biotin blocking reagent, by Vector Laboratories (2 mentions) Cd90 1 his5, by Thermo Fisher Scientific (2 mentions) Cd8β yts156, by BioLegend (2 mentions) Cd4 rm4 5, by Thermo Fisher Scientific (2 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Cryostat, by Thermo Fisher Scientific (1 mentions) Axio scan z1, by Zeiss (1 mentions)

3 protocols using ab26120

1

Quantitative Immunofluorescence Imaging of Intestinal Tissues

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Intestinal tissues were fixed in 4% paraformaldehyde, rehydrated in 20% sucrose, and frozen in OCT media (Sakura). Tissues were cut into 7-8μm sections and treated with ice cold acetone. Sections were treated with biotin-avidin blocking reagent when necessary (Vector labs) and stained with the following biotinylated or directly conjugated antibodies: CD8β (YTS156.7.7, Biolegend), CD4 (RM4-5, eBioscience), CD103 (M290, BD Biosciences), CD90.1 (HIS5.1, eBioscience), Epcam (G8.8, Biolegend), CD11c (HL3, BD Biosciences), B220 (RA3-6B2, eBioscience). Rabbit anti-Yersinia pseudotuberculosis (ab26120, Abcam) and anti-rabbit Dylight 649 (ab96926, Abcam) were used to stain for bacterial antigens. Stained slides were mounted with Prolong Gold antifade reagent (Invitrogen), imaged using a Nikon 90i, and analyzed using Adobe Photoshop software.
The number of OT-I cells/villus was determined by sectioning a ‘Swiss roll’52 (link) of the distal third of the small intesine. Five or more sections/mouse that were at least 400μm apart were stained and imaged. A villus and the underlying submucosa and muscularis were considered a single villus, and the number of OT-I cells in each region was determined. The number of OT-I cells/villus were binned and plotted as the percentage of villi containing a given range of OT-I cells.
Bergsbaken T, & Bevan M.J. (2015). Proinflammatory microenvironments within the intestine regulate differentiation of tissue-resident CD8 T cells responding to infection. Nature immunology, 16(4), 406-414.
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2

Quantitative Immunofluorescence Imaging of Intestinal Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Intestinal tissues were fixed in 4% paraformaldehyde, rehydrated in 20% sucrose, and frozen in OCT media (Sakura). Tissues were cut into 7-8μm sections and treated with ice cold acetone. Sections were treated with biotin-avidin blocking reagent when necessary (Vector labs) and stained with the following biotinylated or directly conjugated antibodies: CD8β (YTS156.7.7, Biolegend), CD4 (RM4-5, eBioscience), CD103 (M290, BD Biosciences), CD90.1 (HIS5.1, eBioscience), Epcam (G8.8, Biolegend), CD11c (HL3, BD Biosciences), B220 (RA3-6B2, eBioscience). Rabbit anti-Yersinia pseudotuberculosis (ab26120, Abcam) and anti-rabbit Dylight 649 (ab96926, Abcam) were used to stain for bacterial antigens. Stained slides were mounted with Prolong Gold antifade reagent (Invitrogen), imaged using a Nikon 90i, and analyzed using Adobe Photoshop software.
The number of OT-I cells/villus was determined by sectioning a ‘Swiss roll’52 (link) of the distal third of the small intesine. Five or more sections/mouse that were at least 400μm apart were stained and imaged. A villus and the underlying submucosa and muscularis were considered a single villus, and the number of OT-I cells in each region was determined. The number of OT-I cells/villus were binned and plotted as the percentage of villi containing a given range of OT-I cells.
Bergsbaken T, & Bevan M.J. (2015). Proinflammatory microenvironments within the intestine regulate differentiation of tissue-resident CD8 T cells responding to infection. Nature immunology, 16(4), 406-414.
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3

Visualizing Y. pseudotuberculosis in Spleen

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Spleen from infected animals were harvested 5 days after infection and fixed directly in 1% PFA (paraformaldehyde) at 4°C overnight. Fixed tissues were washed extensively using PBS, incubated overnight in 30% sucrose at 4°C, and embedded in optimum cutting temperature blocks. Sections (8 μM) were prepared using cryostat (Thermo Fisher Scientific). Sections were blocked and incubated with anti–Y. pseudotuberculosis (1:4000; ab26120, Abcam) for 1 hour at room temperature, followed by secondary antibody (1:500; Thermo Fisher Scientific, goat anti-rabbit 594) and 4′,6-diamidino-2-phenylindole for 30 min. Immunofluorescence of fixed tissues were scanned using Zeiss Axio Scan.Z1.
Demarco B., Grayczyk J.P., Bjanes E., Le Roy D., Tonnus W., Assenmacher C.A., Radaelli E., Fettrelet T., Mack V., Linkermann A., Roger T., Brodsky I.E., Chen K.W, & Broz P. (2020). Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality. Science Advances, 6(47), eabc3465.
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