Cells were lysed at 4 °C in RIPA buffer supplemented with protease and phosphatase inhibitors. Equal loads of 30 μg of proteins were electrophoretically separated using SDS/polyacrylamide gels and then transferred to the PVDF membrane (Millipore, Bedford, MA, USA). After blocking with 5% nonfat milk, the membrane was made to react with specific antibodies (ALDH1A3, Abcam #ab129825, 1:1000; STAT3, Cell Signaling #4904, 1:1000; p-STAT3 (Try705), Cell Signaling #9145, 1:1000; STAT1, Santa Cruz #sc-417, 1:1000; p-STAT1 (Try701), Cell Signaling #9167, 1:1000; β-actin, Sigma-Aldrich #A2228, 1:1000) overnight at 4 °C and then incubated with horseradish peroxidase-conjugated secondary antibody for 1 h. Blots were visualized using an ECL-Plus detection kit (PerkinElmer Life Sciences, Boston, MA, USA).
P stat1 try701
Manufactured by Cell Signaling Technology
Sourced in United States
P-STAT1 (Try701) is an antibody that specifically recognizes the phosphorylated form of STAT1 protein at the Tyr701 residue. It can be used to detect the activation of the STAT1 signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Ecl plus detection kit, by PerkinElmer (2 mentions)
Stat1, by Santa Cruz Biotechnology (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
β actin, by Merck Group (2 mentions)
P stat3 try705, by Cell Signaling Technology (1 mentions)
Stat3, by Cell Signaling Technology (1 mentions)
Lamin a c, by GeneTex (1 mentions)
P nf κb ser536, by Cell Signaling Technology (1 mentions)
γh2ax, by Merck Group (1 mentions)
Sting, by Cell Signaling Technology (1 mentions)
Nf κb, by Cell Signaling Technology (1 mentions)
Pd l1, by GeneTex (1 mentions)
2 protocols using p stat1 try701
1
Western Blot Analysis of ALDH1A3, STAT1, and STAT3
2
Western Blot Analysis of Signaling Proteins
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Cells were lysed at 4°C in RIPA buffer supplemented with protease and phosphatase inhibitors. Total lysates (containing 30 µg of protein) were separated through sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto PVDF membranes (Millipore, Bedford, MA, USA). After they had been blocked with 5% nonfat milk, the membranes were probed with specific antibodies [γH2AX, Millipore #05-636-I; p-IRF-3 (ser396), Invitrogen #70012; IRF-3, Cell Signaling #4302; STING, Cell Signaling #13647; NF-κB, Cell Signaling #8242; p-NF-κB (ser536), Cell Signaling #3033; Lamin A/C, Genetex #GTX101127; p-STAT1 (Try701), Cell Signaling #9167; STAT1, Santa Cruz #sc-417; PD-L1, Genetex #GTX104763, Abcam #ab269674; β-actin, Millipore #MAB1501] at 4°C overnight and then incubated with the horseradish peroxidase-conjugated secondary antibody for 1 h. All signals were visualized using the ECL-Plus detection kit (PerkinElmer Life Sciences, Boston, MA, USA).
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