Ca2+ transients reported by GCaMP6s were imaged over 6 weeks post AAV injection (ISOI/2p calibration experiments) or at two developmental time points, P20 and P36, in GCaMP6s expressing transgenic mice, using a custom-built two-photon microscope using galvanometer-based scan mirrors (6mm diameter, Cambridge Technologies) with a 16 x magnification and 0.8 numerical aperture water immersion objective (Nikon) and a large aperture collection pathway with GaAsP photomultiplier tubes (Hamamatsu). Frame scans (1 frame/s or 15.6 frames/s) were acquired using ScanImage51 (link).
0.8 numerical aperture water immersion objective
Manufactured by Nikon
The 0.8 numerical aperture water immersion objective is a specialized lens designed for microscopy applications. It has a numerical aperture of 0.8, which allows for high-resolution imaging and the capture of fine details. This objective is intended for use with water-based sample preparations, providing optimal optical performance in a wet environment.
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Lab products found in correlation
2 protocols using 0.8 numerical aperture water immersion objective
1
Longitudinal Calcium Imaging in Mice
2
Longitudinal Calcium Imaging in Mice
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