A culture assay was performed to determine the efficacy of the combination of ABZ and ATV. The protoscoleces obtained were cultured in Connaught Medical Research Laboratories 1066 medium (Gibco, Grand Island, NY, USA) containing 0.5% (w/v) yeast extract (Difco Laboratories, Detroit, MI, USA), 23 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 0.5% (w/v) D (+)-glucose, 0.4 mM sodium taurocholate (Wako Pure Chemical Industries, Osaka, Japan), 57 mM sodium hydrogen carbonate, 2 mM L-glutamine (Gibco), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco). Half of the medium was changed on day 3. For anaerobic cultures, six-well plates were sealed in plastic containers with oxygen-detecting agents and oxygen scavengers (Aneromeito®, Nissui Pharmaceutical, Tokyo, Japan) to maintain the oxygen concentration under 0.3% at 37 °C. The protoscoleces were treated with ABZ (Tokyo Chemical Industry, Tokyo, Japan) and/or ATV (Tokyo Chemical Industry) at a final concentration of 50 μM in the culture medium, and the duration of parasite elimination was determined. The control group was supplemented with 0.5% (v/v) dimethyl sulfoxide (DMSO), and all conditions were assayed in triplicate. The viability of protoscoleces was determined by microscopic observation of more than 170 protoscoleces per well using the trypan blue exclusion test.
1066 medium
Manufactured by Thermo Fisher Scientific
Sourced in United States
1066 medium is a cell culture medium designed for the growth and maintenance of various cell types. It provides the necessary nutrients, growth factors, and a buffered environment to support cell proliferation and viability. The detailed composition and specifications of 1066 medium are available upon request.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Yeast extract, by BD (1 mentions)
Sodium taurocholate, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Collagenase 4, by Merck Group (1 mentions)
Bilirubin, by Merck Group (1 mentions)
2 protocols using 1066 medium
1
Evaluating ABZ and ATV Combination Against Protoscoleces
2
Islet Preservation with Antioxidants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All animal experiments were approved by the Animal Care and Use Committee at the Wenzhou Medical University. Mice were anesthetized before laparotomy. The pancreas was distended with Hank's Balanced Salt Solution containing collagenase IV (Sigma) through the common bile duct and subsequently excised. The isolated pancreatic tissue was then digested by collagenase IV at 37°C. Hank solution was used to remove the residual collagenase IV. Then, islets were further purified by density gradient centrifugation and collected by hand-pick under a microscope. Isolated islets were cultured for 6 hours in 1066 medium (Gibco) containing 10% fetal bovine serum (Sigma) and 1% antibiotics at 37°C before preservation.
During the preservation, the isolated islets were cultured in complete medium with or without antioxidants (Bilirubin or procyanidin [OPC]) at 4°C for a predetermined time. Bilirubin (Sigma) was dissolved with dimethyl sulfoxide as 5 mg/mL, then diluted to different concentration with 1066 medium for islet culture. The hypothermic preserved islets were warm up to 37°C before evaluation.
During the preservation, the isolated islets were cultured in complete medium with or without antioxidants (Bilirubin or procyanidin [OPC]) at 4°C for a predetermined time. Bilirubin (Sigma) was dissolved with dimethyl sulfoxide as 5 mg/mL, then diluted to different concentration with 1066 medium for islet culture. The hypothermic preserved islets were warm up to 37°C before evaluation.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!