Macs vitalvirus hiv isolation kit

Manufactured by Miltenyi Biotec

The µMACS™ VitalVirus HIV Isolation Kit is a laboratory equipment product designed for the isolation of HIV from biological samples. The kit utilizes magnetic beads coated with antibodies specific to HIV particles, enabling the efficient capture and separation of the virus from the sample matrix.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) 0.45 μm filter, by Thermo Fisher Scientific (2 mentions)

2 protocols using macs vitalvirus hiv isolation kit

Isolation and purification of HIV-1 virions

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The cell culture media containing HIV-1 particles were centrifuged at 1,200 × g for 5 min. The supernatants were passed through a 0.45-μm filter (ThermoFisher) and layered onto 20% sucrose for a 2-h ultracentrifugation at 25,000 × g at 4 °C. The virion pellets were subsequently isolated using anti-CD44 microbeads with a magnetic-based method according to the manufacturers’ instructions (µMACS™ VitalVirus HIV Isolation Kit, Miltenyi Biotec). The purified virions were lysed with RIPA buffer (ThermoFisher) and subjected to SDS-PAGE for western blotting as previously described (20 (link)).

Pang H., Ouyang J., Yang Z., Shang H, & Liang G. (2023). Urokinase plasminogen activator surface receptor restricts HIV-1 replication by blocking virion release from the cell membrane. Proceedings of the National Academy of Sciences of the United States of America, 120(3), e2212991120.

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Isolation of HIV-1 Virions

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cell culture media containing HIV-1 particles were centrifuged at 1,200 × g for 5 min. The supernatants were passed through a 0.45-μm filter (Thermo Fisher) and layered on 20% sucrose for a 2-h ultracentrifugation at 25,000 × g at 4 °C. The virion pellets were subsequently isolated using anti-CD44 microbeads with a magnetic-based method according to the manufacturers’ instructions (µMACS VitalVirus HIV Isolation Kit; Miltenyi Biotec). The purified virions were lysed with RIPA buffer (Thermo Fisher) and subjected to SDS-PAGE for Western blotting.

Wang S., Zhao L., Zhang X., Zhang J., Shang H, & Liang G. (2022). Neuropilin-1, a myeloid cell-specific protein, is an inhibitor of HIV-1 infectivity. Proceedings of the National Academy of Sciences of the United States of America, 119(2), e2114884119.

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