Escherichia coli K12 MG1655 or E. coli Nissle 1917 carrying a P16Lux plasmid [47 (link)] was grown aerobically at 37°C in Luria-Bertani (LB) medium with 300 µg/ml Erythromycin (Sigma-Aldrich). Staphylococcus aureus Newman (ATCC 25904) was grown aerobically at 37°C in Todd-Hewitt broth (Sigma-Aldrich). Bifidobacterium longum has grown anaerobically at 37°C for 24 h in De Man, Ragosa, Sharpe (MRS) medium (Sigma-Aldrich). Lactobacillus amylophilus (ATCC® 49845™) was grown in MRS medium (Sigma-Aldrich) at 30°C in 5% CO2 for 24 h. Bacteroides thetaiotaomicron (ATCC®29741™) was grown anaerobically at 37°C for 24 h in Fastidious Anaerobe Broth (FAB) medium (NEOGEN, Lancashire, UK). Bacterial cultures were harvested by centrifugation and suspended in PBS. A 1 ml aliquot of the suspension was used to count colony-forming units (CFUs) by retrospective plating. The rest was resuspended in neutral buffered formalin and left to fix for 18 h at room temperature (RT).
Lactobacillus amylophilus
Manufactured by American Type Culture Collection
Sourced in United Kingdom
Lactobacillus amylophilus is a lactic acid bacterium. It has the ability to hydrolyze starch.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lactobacillus amylophilus
1
Cultivation of common bacteria for research
Check if the same lab product or an alternative is used in the 5 most similar protocols
Escherichia coli K12 MG1655 or E. coli Nissle 1917 carrying a P16Lux plasmid [47 (link)] was grown aerobically at 37°C in Luria-Bertani (LB) medium with 300 µg/ml Erythromycin (Sigma-Aldrich). Staphylococcus aureus Newman (ATCC 25904) was grown aerobically at 37°C in Todd-Hewitt broth (Sigma-Aldrich). Bifidobacterium longum has grown anaerobically at 37°C for 24 h in De Man, Ragosa, Sharpe (MRS) medium (Sigma-Aldrich). Lactobacillus amylophilus (ATCC® 49845™) was grown in MRS medium (Sigma-Aldrich) at 30°C in 5% CO2 for 24 h. Bacteroides thetaiotaomicron (ATCC®29741™) was grown anaerobically at 37°C for 24 h in Fastidious Anaerobe Broth (FAB) medium (NEOGEN, Lancashire, UK). Bacterial cultures were harvested by centrifugation and suspended in PBS. A 1 ml aliquot of the suspension was used to count colony-forming units (CFUs) by retrospective plating. The rest was resuspended in neutral buffered formalin and left to fix for 18 h at room temperature (RT).
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Escherichia coli K12 MG1655 or E. coli Nissle 1917 carrying a P16Lux plasmid [47 (link)] was grown aerobically at 37°C in Luria-Bertani (LB) medium with 300 µg/ml Erythromycin (Sigma-Aldrich). Staphylococcus aureus Newman (ATCC 25904) was grown aerobically at 37°C in Todd-Hewitt broth (Sigma-Aldrich). Bifidobacterium longum has grown anaerobically at 37°C for 24 h in De Man, Ragosa, Sharpe (MRS) medium (Sigma-Aldrich). Lactobacillus amylophilus (ATCC® 49845™) was grown in MRS medium (Sigma-Aldrich) at 30°C in 5% CO2 for 24 h. Bacteroides thetaiotaomicron (ATCC®29741™) was grown anaerobically at 37°C for 24 h in Fastidious Anaerobe Broth (FAB) medium (NEOGEN, Lancashire, UK). Bacterial cultures were harvested by centrifugation and suspended in PBS. A 1 ml aliquot of the suspension was used to count colony-forming units (CFUs) by retrospective plating. The rest was resuspended in neutral buffered formalin and left to fix for 18 h at room temperature (RT).
2
Cultivation and Quantification of Diverse Bacterial Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
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E. coli K12 MG1655 or E. coli Nissle 1917 carrying a P16Lux plasmid [50 (link)] were grown aerobically at 37 °C in Luria-Bertani (LB) medium with 300 μg/ml Erythromycin (Sigma-Aldrich). Staphylococcus aureus Newman (ATCC 25904) was grown aerobically at 37 °C in Todd-Hewitt broth (Sigma-Aldrich). Bifidobacterium longum 35624 was grown anaerobically at 37 °C for 24 h in MRS medium (Sigma-Aldrich). Lactobacillus amylophilus (ATCC® 49845™) was grown in MRS medium (Sigma-Aldrich) at 30 °C in 5 % CO2 for 24 h. Bacteroides thetaiotaomicron (ATCC®29741™) was grown anaerobically at 37 °C for 24 h in FAB medium (NEOGEN, Lancashire, UK). Bacterial cultures were harvested by centrifugation and suspended in PBS. A 1 ml aliquot of the suspension was used for to count colony-forming units (CFU) by retrospective plating. The rest was resuspended in Neutral Buffered Formalin and left to fix for 18 h at room temperature (RT).
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