Automatic syringe pump

Manufactured by KD Scientific

Sourced in United States

The Automatic Syringe Pump is a laboratory device designed to precisely control the flow rate and volume of liquids dispensed from a syringe. It is capable of accurately delivering small volumes of fluids at a consistent rate, making it a useful tool for various scientific and research applications.

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Lab products found in correlation

D3305, by Thermo Fisher Scientific (1 mentions) D1842, by Thermo Fisher Scientific (1 mentions) C57bl 6 mice, by Charles River Laboratories (1 mentions) Low concentration tuning mix solution, by Agilent Technologies (1 mentions) Data analysis software version 4, by Bruker (1 mentions) Microtof q 2 mass spectrometer, by Bruker (1 mentions)

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Syringe pump

4 protocols using automatic syringe pump

Tracing Brain Perivascular Clearance

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Three kDa fluorescein isothiocyanate (FITC)-labelled dextran (3 k-FITC, D3305, Invitrogen) and 40 kDa tetramethylrhodamine (TMR)-labelled dextran (40 k-TMR, D1842, Invitrogen) was dissolved in aCSF and delivered through cisterna magna (CM). 3 k-FITC has a molecular weight similar to that of the Aβ monomer and is known to have access to the brain independent of AQP4. 40 k-TMR is suitable to examine the perivascular CSF influx affected by AQP4. During the experiment, the heart rate was monitored with a set of three platinum needle electrodes connected to the data acquisition system iX228s (Iworx). The head was fixed to the stereotactic frame, and the head and neck were positioned at 120°. After incising the dorsal neck skin, the neck muscles were exposed with a retractor to access the atlanto-occipital membrane overlying the CM. A disposable 30 G needle was carefully inserted and fixed with a cyanoacrylate bond while avoiding contact with the medulla or cerebellum. The solution was delivered by an automatic syringe pump (KD Scientific) for 5 min at 2 µl/min and cardiac perfusion was performed 30 min after the injection [48 (link)].

Kim S.H., Ahn J.H., Yang H., Lee P., Koh G.Y, & Jeong Y. (2020). Cerebral amyloid angiopathy aggravates perivascular clearance impairment in an Alzheimer’s disease mouse model. Acta Neuropathologica Communications, 8, 181.

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Longitudinal In Vivo Imaging of Mouse Brain

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All animal studies were performed at the Duke Center for In Vivo Microscopy and were approved by the Duke Institutional Animal Use and Care Committee. The protocols adhered to the NIH Guide for the Care and Use of Laboratory Animals.
C57BL/6 mice (n=5, Charles River Laboratories, Wilmington, MA) were imaged longitudinally over a 17-week course (3, 5, 7, 9, 13, 17 weeks). Animals were provided with free access to water before imaging studies. While imaging, the animals were anesthetized under isoflurane and were breathing freely. Three-dimensional printing (Stratasys Dimension, Eden Prairie, MN) was used to make custom parts for the nose cone and for proper positioning of the animal inside the cryogenic surface coil, which was designed for brain imaging.
Gadolinium-based contrast agent (gadofosveset trisodium, Lantheus Medical Imaging, Billerica, MA) was injected as a bolus via tail vein catheter with a dose of 0.03 mmol/kg (similar to clinical dose) and at a rate of approximately 0.35mL/min. Contrast was injected using an automatic syringe pump (KD Scientific, Holliston, MA).

Xie L., Subashi E., Qi Y., Knepper M.A, & Johnson G.A. (2014). 4D MRI of renal function in the developing mouse. NMR in biomedicine, 27(9), 1094-1102.

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Spectroscopic Characterization of Compounds

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The structures were confirmed by melting point (mp), proton (¹H NMR) and carbon (¹³C NMR) nuclear magnetic resonance spectroscopy. The mps were determined with a Microquimica MGAPF-301 apparatus (Microquímica Equipamentos Ltda., Palhoça, SC, Brazil) and were uncorrected. NMR (¹H and ¹³C) spectra were recorded on a Varian Oxford AS-400 (Agilent Technologies, Santa Clara, CA, USA) (400 MHz) or on a Bruker Ac-200F instrument (Bruker, Billerica, MA, USA) (200 MHz), using tetramethylsilane as internal standard. ¹H NMR spectra revealed that all the structures were geometrically pure and E configured (JHα–Hβ =16 Hz). High-resolution mass spectra (HRMS) were recorded on a micrOTOF-QII mass spectrometer (Bruker) equipped with an automatic syringe pump (KD Scientific, Holliston, MA, USA) for sample injection (constant flow of 3 μL/minute) by positive mode of electron spray ionization technique (4.5 kV and 180°C). The instrument was calibrated in the range of 50–3,000 m/z using an internal calibration standard (low concentration tuning mix solution) (Agilent Technologies). An acetonitrile/methanol mixture was used as solvent of the compounds. Data were processed via Bruker Data Analysis Software (version 4.0).

Winter E., Gozzi G.J., Chiaradia-Delatorre L.D., Daflon-Yunes N., Terreux R., Gauthier C., Mascarello A., Leal P.C., Cadena S.M., Yunes R.A., Nunes R.J., Creczynski-Pasa T.B, & Di Pietro A. (2014). Quinoxaline-substituted chalcones as new inhibitors of breast cancer resistance protein ABCG2: polyspecificity at B-ring position. Drug Design, Development and Therapy, 8, 609-619.

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Fluorescent Tracer Injection in Somatosensory Cortex

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A solution of 3 k-FITC dissolved in aCSF at 0.1% concentration was injected into the brain parenchyma. The solution was loaded in a glass capillary with a diameter of less than 60 µm connected to an automatic syringe pump (KD Scientific) to deliver the solution at 0.1 µl/min for 5 min. The injection site was the left somatosensory cortex (AP: − 0.5 mm, ML: − 2.2 mm, DV: − 1.3 mm). To prevent reflux, the glass capillary was left for 10 min after injection, and cardiac perfusion was performed 30 min after intra parenchymal injection.

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