The MTT assay was performed as described in the previous study [25 (link)]. In brief, the cells in the logarithmic growth phase were seeded in 96 plates at a density of 5 × 103 cells per well. After 24 h of cell attachment, the cells were treated with different concentrations of compounds and 0.1% DMSO, respectively, for 72 h, with six replicates for each treatment group. Then 10 μL of MTT solution (5 mg/mL) was added to each well and the cells were returned to the incubator and incubated for a further 4 h. At the end of the incubation, the entire culture solution was gently aspirated from the cells and 100 μL of DMSO solution was added to each well. The solution in the 96-well plate was then shaken well and mixed, and the absorbance of each well was measured at 570 nm by using a hybrid plate reader (Bio Tek, Winooski, VT, USA). GraphPad Prism 8.0 software was used to fit the IC50 for compound 3 inhibition of cell viability.
Hybrid plate reader
Manufactured by Agilent Technologies
Sourced in United States
The Hybrid Plate Reader is a versatile laboratory instrument designed for high-performance multimode detection. It combines the capabilities of a microplate reader, a fluorescence reader, and a luminescence reader in a single, compact unit.
Automatically generated - may contain errors
6 protocols using hybrid plate reader
1
MTT Assay for Cell Viability Analysis
2
PAXgene RNA Extraction Protocol
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PAXgene® Blood RNA tubes were thawed overnight (16–20 h) at room temperature prior to RNA extraction. The PAXgene Blood RNA kit (Pre-Analytix) was used to purify/extract intracellular RNA, per manufacturer’s instructions. RNA concentration and quality was determined by absorbance using a Take3 Trio Microplate (BioTek®) read on a Syntek Hybrid Plate Reader and analyzed using Gen5 (BioTek) software. A260/A280 values between 1.8 and 2.2 were considered acceptable RNA quality.
3
PAXgene Blood RNA Extraction Protocol
4
PAXgene Blood RNA Extraction Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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PAXgene® Blood RNA tubes were thawed overnight (16 – 20 h) at room temperature prior to RNA extraction. The PAXgene Blood RNA kit (Pre-Analytix) was used to purify/extract intracellular RNA, per manufacturer’s instructions. RNA concentration and quality was determined by absorbance using a Take3 Trio Microplate (BioTek®) read on a Syntek Hybrid Plate Reader and analyzed using Gen5 (BioTek®) software. A260/A280 values between 1.8 and 2.2 were considered acceptable RNA quality.
5
Whole Blood Collection and RNA Extraction for TBI
6
PAXgene Blood RNA Extraction Protocol
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