Calcein acetoxymethyl ester calcein am

Manufactured by Solarbio

Sourced in China

Calcein acetoxymethyl ester (Calcein AM) is a cell-permeant fluorescent dye that is commonly used as a viability indicator in cell-based assays. It is a non-fluorescent compound that becomes fluorescent upon hydrolysis by intracellular esterases, which occurs in live cells. The fluorescence of the hydrolyzed product can be measured to assess cell viability.

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Lab products found in correlation

Propidium iodide, by Solarbio (2 mentions) Tannic acid, by Sinopharm (1 mentions) Alkaline phosphatase alp, by Sinopharm (1 mentions) Rhodamine b isothiocyanate, by Sinopharm (1 mentions) Er tracker, by Thermo Fisher Scientific (1 mentions) 5 n ethyl n isopropyl amiloride eipa, by Merck Group (1 mentions) Isopropanol ipa, by Sinopharm (1 mentions) Mitotracker, by Thermo Fisher Scientific (1 mentions) Trypsin ethylenediaminetetraacetic acid trypsin edta, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline ph 7, by Thermo Fisher Scientific (1 mentions) Lysotracker, by Thermo Fisher Scientific (1 mentions) Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Calcein-AM (27 citations) Calcein (3 citations)

2 protocols using calcein acetoxymethyl ester calcein am

Polyethylene Scaffold Biocompatibility

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Tissue-culture polyethylene (CPE) and ordinary polyethylene (PE) were provided by NEST Biotechnology Co., Ltd., Wuxi, China. Hydroxyl-terminated poly(N-isopropylacrylamide) (PNIPAAm-OH) was prepared in our lab (Scheme S1a). Tannic acid (TA, ≥98%), rhodamine B isothiocyanate (BRITC, 99%), 3-aminopropylthiethoxysilane (APTES, 98%), alkaline phosphatase (ALP, 98%), an ALP assay kit, and DMEM were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China. Mouse fibroblast cells (L929 cells), phosphate buffer saline (PBS, 99.9%, pH = 7.4), and fetal bovine serum (FBS, 97%) were obtained from Guangzhou Oricell Biotechnology Co., Ltd., Guangzhou, China. Calcein acetoxymethyl ester (Calcein AM) and propidium iodide (PI) were obtained from Solarbio, Beijing, China. Carbon dioxide (CO₂, 99.999%) was supplied by Guangzhou Spectral Source Gas Co., Ltd., Guangzhou, China.

Xu J., Liu X., Liang P., Yuan H, & Yang T. (2024). In Situ Preparation of Tannic Acid-Modified Poly(N-isopropylacrylamide) Hydrogel Coatings for Boosting Cell Response. Pharmaceutics, 16(4), 538.

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Biomaterial Synthesis and Cellular Uptake

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GA (purity: 99%) was obtained from Feiyu Biological Co., Ltd (Jiangsu, China). Boron powder, purchased from Maclin (Shanghai, China), was stored in an argon glove box. Isopropanol (IPA) and ethanol (anhydrous grade) were obtained from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). PEG-NH₂ (MW = 2000) was provided by Xi'an Regal Biotech Co., Ltd. Ultrapure water (18.25 MΩ.cm, 25 °C) was used to prepare all solutions. Phosphate-buffered saline (PBS, pH 7.4), fetal bovine serum (FBS), Dulbecco's modified eagle medium (DMEM), and trypsin-ethylenediaminetetraacetic acid (Trypsin-EDTA) were obtained from Thermo Fisher Scientific Inc. (USA). Cell counting kit-8 (CCK8) was purchased from Dojindo Laboratories (Japan). Cy5.5-PEG-NH₂ and FITC-PEG-NH₂ were obtained from Ruixi Biological Co., Ltd. (Shanxi, China). Propidium iodide (PI) and calcein acetoxymethyl ester (calcein-AM) were obtained from Solarbio Science & Technology Co., Ltd. (China). Methyl-β-cyclodextrin (MβCD), chlorpromazine (CPZ), hypertonic sucrose, and 5-(N-ethyl- N-isopropyl)-amiloride (EIPA) were purchased from Sigma Aldrich (St. Louis, MO, USA). 4′,6-diamidino-2-phenylindole (DAPI), Lyso Tracker, ER Tracker, and Mito Tracker were obtained from Molecular Probes Inc. (OR, USA).

Zhao Y., Liu N., Liu P., Fan T., Ma R., Hong H., Chen X., Xie Z., Zhang H., Wang Q, & Chen T. (2022). Robust boron nanoplatform provokes potent tumoricidal activities via inhibiting heat shock protein. Asian Journal of Pharmaceutical Sciences, 17(5), 728-740.

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