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Np80 uv vis spectrophotometer

Manufactured by Implen
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Sourced in Germany

The NP80 UV–Vis spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light in the ultraviolet and visible spectrum. It is used to quantify the concentration of specific substances in a sample by analyzing the absorption of light at specific wavelengths.

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Lab products found in correlation

Hifair 2 1st stand cdna synthesis supermix, by Yeasen (1 mentions) Gdna digester, by Yeasen (1 mentions) Rnaiso plus, by Takara Bio (1 mentions)

2 protocols using np80 uv vis spectrophotometer

1

RNA Extraction and cDNA Synthesis

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Total RNA was extracted from three pools of tissues, different stages, and survived induction nymphs using RNAiso Plus (Takara, Shiga, Japan), following the manufacturer’s instructions. DNA contamination was removed by treating RNA extraction products with gDNA digester (Yeasen, Shanghai, China). The quality and concentration of RNA samples were checked spectrophotometrically at 260/280 nm using an NP80 UV–Vis spectrophotometer (IMPLEN, Munich, Germany). One microgram of total RNA was reverse transcribed to first-strand cDNA by using Hifair™ II 1st Stand cDNA Synthesis SuperMix (Yeasen, Shanghai, China).
Li Z., Cai T., Qin Y., Zhang Y., Jin R., Mao K., Liao X., Wan H, & Li J. (2020). Transcriptional Response of ATP-Binding Cassette (ABC) Transporters to Insecticide in the Brown Planthopper, Nilaparvata lugens (Stål). Insects, 11(5), 280.
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2

Encapsulation Efficiency of Polyphenol Nanoparticles

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The encapsulation efficiency (% EE) was analyzed by comparing the difference in absorbance at A296nm between free PCA and the supernatant of encapsulated PCA. The nanoparticles samples were prepared as previously described. The samples were centrifuged at 18,000 rpm for 30 min. The supernatant of each sample was then collected, and the absorbance was read at A296nm using an Implen NP80 UV/VIS spectrophotometer. The % EE was calculated using the following equation: % EE=A296 of free PCA − A296 of PCA in supernatantA296 of free PCA × 100%
Yee Kuen C., Galen T., Fakurazi S., Othman S.S, & Masarudin M.J. (2020). Increased Cytotoxic Efficacy of Protocatechuic Acid in A549 Human Lung Cancer Delivered via Hydrophobically Modified-Chitosan Nanoparticles As an Anticancer Modality. Polymers, 12(9), 1951.
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