Thin blood smears from cultured parasites were prepared, air-dried and fixed in a 1:1 acetone:methanol mixture at − 30 °C for 5 min31 (link). Smears were blocked with PBS containing 10% normal goat serum (Invitrogen) at 37 °C for 30 min and immunostained with mouse anti-myc monoclonal antibody (9B11, Cell Signaling Technology) at 1:500 dilution in PBS supplemented with 0.05% Tween-20 and incubated at 4 °C overnight. Double immunostaining of smears was done with rabbit anti-SBP4 at 1:1000 dilution. The smears were incubated with Alexa Fluor 488-conjugated goat anti-mouse or Alexa Fluor 594-conjugated goat anti-rabbit IgG antibody (1:500; Invitrogen) at 37 °C for 30 min. Nuclei were stained by incubation of smears with 1 μg/mL Hoechst 33342 solution. The smears were examined using a confocal laser-scanning microscope (CS-SP5, Leica Micro-system, Wetzlar, Germany).
Cs sp5
Manufactured by Leica
Sourced in Germany
The CS-SP5 is a confocal microscope system manufactured by Leica. It is designed for high-resolution imaging and analysis of biological samples. The system features a modular design and offers advanced optics and imaging capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Normal goat serum (ngs), by Thermo Fisher Scientific (2 mentions)
Alexa fluor 594 conjugated goat anti rabbit igg antibody, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 488 conjugated goat anti mouse, by Thermo Fisher Scientific (2 mentions)
Mouse monoclonal anti myc antibody, by Cell Signaling Technology (1 mentions)
Mouse anti myc monoclonal antibody 9b11, by Cell Signaling Technology (1 mentions)
2 protocols using cs sp5
1
Immunostaining of Cultured Parasite Smears
2
Immunostaining of Cultured Parasites
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Thin blood smears from cultured parasites were prepared, air-dried and fixed in a 1:1 acetone:methanol mixture at -30°C for 5 min 29 . Smears were blocked with PBS containing 10% normal goat serum (Invitrogen) at 37°C for 30 min and immunostained with mouse anti-myc monoclonal antibody (9B11, Cell Signaling Technology) at 1:500 dilution in PBS supplemented with 0.05% Tween-20 and incubated at 4°C overnight. Double immunostaining of smears was done with rabbit anti-SBP4 at 1:1000 dilution. The smears were incubated with Alexa fluor ® 488conjugated goat anti-mouse or Alexa fluor ® 594-conjugated goat anti-rabbit IgG antibody (1:500; Invitrogen) at 37°C for 30 min. Nuclei were stained by incubation of smears with 1 μg/mL Hoechst 33342 solution. The smears were examined using a confocal laser-scanning microscope (CS-SP5, Leica Micro-system, Wetzlar, Germany).
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