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2 protocols using rabbit anti akt and anti β catenin phospho forms

1

Functional Blocking of Leukocyte Adhesion

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Function blocking anti-human ICAM-1 (15.2) from Serotec (Raleigh, NC), anti-mouse ICAM-1mAb (YN1/1.7.4), anti-Ly-6G conjugated to Alexa 488 and anti-mouse MHC Class-I (W6/32) from eBioscience (San Diego, CA), anti-CD11b/CD18 mAb (CBRM1/29) purified in-house as previously described,59 (link) Rabbit anti-Akt and anti-β-catenin phospho forms from Cell Signaling (Danvers, MA), anti-Mouse-Alexa488 (Carlsbad, CA), anti-human MHC Class-I (1.B.548) and Rabbit anti-Ki67 from Abcam (Cambridge, MA), Isotype control IgG1 from BD Biosciences (San Jose, CA), HRP-conjugated anti-mouse and anti-rabbit IgGs from Jackson Immunoresearch (West Grove, PA). Click-iT® EdU Alexa Fluor® 488 Imaging Kit Molecular Probes (Eugene, Oregon), Akt inhibitor VIII (Billerica, Massachusetts, EMD Millipore), ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid), L-mimosine, HBSS with Ca2+ and Mg2+ (HBSS+) and HBSS without Ca2+ and Mg2+ (HBSS-), DMEM including media supplements as well as chemotactic peptide fMLF, mouse IFNγ and TNFα from Sigma (St Louis, MO).
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2

Functional Blocking of Leukocyte Adhesion

Check if the same lab product or an alternative is used in the 5 most similar protocols
Function blocking anti-human ICAM-1 (15.2) from Serotec (Raleigh, NC), anti-mouse ICAM-1mAb (YN1/1.7.4), anti-Ly-6G conjugated to Alexa 488 and anti-mouse MHC Class-I (W6/32) from eBioscience (San Diego, CA), anti-CD11b/CD18 mAb (CBRM1/29) purified in-house as previously described,59 (link) Rabbit anti-Akt and anti-β-catenin phospho forms from Cell Signaling (Danvers, MA), anti-Mouse-Alexa488 (Carlsbad, CA), anti-human MHC Class-I (1.B.548) and Rabbit anti-Ki67 from Abcam (Cambridge, MA), Isotype control IgG1 from BD Biosciences (San Jose, CA), HRP-conjugated anti-mouse and anti-rabbit IgGs from Jackson Immunoresearch (West Grove, PA). Click-iT® EdU Alexa Fluor® 488 Imaging Kit Molecular Probes (Eugene, Oregon), Akt inhibitor VIII (Billerica, Massachusetts, EMD Millipore), ABTS (2,2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid), L-mimosine, HBSS with Ca2+ and Mg2+ (HBSS+) and HBSS without Ca2+ and Mg2+ (HBSS-), DMEM including media supplements as well as chemotactic peptide fMLF, mouse IFNγ and TNFα from Sigma (St Louis, MO).
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