Normal non-immortalized epithelial human fetal colon cells (FHC) were purchased from American Type Culture Collection (ATCC, Manassas, VA) and maintained in the prescribed media. Briefly, 90% Dulbecco's Modified Eagle Medium: Nutrient mixture F-12 (DMEM:F-12)media (ATCC) and 10% fetal bovine serum (FBS) (Mediatech, Manassa, VA), supplemented with 25 mM HEPESL (Life Technologies, Grand Island, NY); 10 ng/ml cholera toxin (Sigma Aldrich, St. Louis, MO); 0.005 mg/ml insulin (Eli Lilly and Company, Indianapolis, Indiana); 0.005 mg/ml transferrin and 100 ng/ml (equal 0.2 μM) hydrocortisone(HC) (Sigma Aldrich); 100 U/ml penicillin and 100 μg/ml streptomycin (Life Technologies). Cells were maintained at 37°C in 5% CO2, 95% O2 in a humidified incubator. Human adult NCM460 colonocytes derived from normal colon were provided by INCELL (San Antonio, TX) and cultured in M3:Base A ™ (M3A) media (INCELL), supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. The cells were cultured at 37 °C in 5% CO2, and 95% O2 in a humidified incubator.
Hepesl
Manufactured by Thermo Fisher Scientific
The HEPESL is a laboratory equipment product designed for use in various scientific applications. It serves as a buffer solution with a pH range suitable for maintaining optimal environmental conditions for cell cultures and other biological experiments. The HEPESL provides a consistent and reliable pH control mechanism to support the growth and maintenance of cells and other biological samples.
Automatically generated - may contain errors
Lab products found in correlation
M3 base a m3a media, by INCELL (2 mentions)
Hydrocortisone hc, by Merck Group (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Cholera toxin, by Merck Group (2 mentions)
Transferrin, by Merck Group (2 mentions)
Penicillin, by INCELL (2 mentions)
Streptomycin, by INCELL (2 mentions)
Dmem f12 media, by American Type Culture Collection (2 mentions)
Insulin, by Eli Lilly (2 mentions)
2 protocols using hepesl
1
Culture of Human Colon Epithelial Cells
2
Culture of Human Colon Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Normal non-immortalized epithelial human fetal colon cells (FHC) were purchased from American Type Culture Collection (ATCC, Manassas, VA) and maintained in the prescribed media. Briefly, 90% Dulbecco's Modified Eagle Medium: Nutrient mixture F-12 (DMEM:F-12)media (ATCC) and 10% fetal bovine serum (FBS) (Mediatech, Manassa, VA), supplemented with 25 mM HEPESL (Life Technologies, Grand Island, NY); 10 ng/ml cholera toxin (Sigma Aldrich, St. Louis, MO); 0.005 mg/ml insulin (Eli Lilly and Company, Indianapolis, Indiana); 0.005 mg/ml transferrin and 100 ng/ml (equal 0.2 μM) hydrocortisone(HC) (Sigma Aldrich); 100 U/ml penicillin and 100 μg/ml streptomycin (Life Technologies). Cells were maintained at 37°C in 5% CO2, 95% O2 in a humidified incubator. Human adult NCM460 colonocytes derived from normal colon were provided by INCELL (San Antonio, TX) and cultured in M3:Base A ™ (M3A) media (INCELL), supplemented with 10% FBS, 100 U/ml penicillin and 100 μg/ml streptomycin. The cells were cultured at 37 °C in 5% CO2, and 95% O2 in a humidified incubator.
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