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4 protocols using neocuproine

1

Spectrophotometric Analysis of Antioxidant Compounds

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Acetic acid (99.5%), aluminum chloride hexahydrate, copper(II) chloride dihydrate, disodium hydrogen phosphate dihydrate, 96% ethanol, 36% hydrochloric acid, iron(III) chloride hexahydrate, methanol, phosphoric acid, potassium persulfate, potassium dihydrogen phosphate, potassium hexacyanoferrate(III), sodium acetate anhydrous, sodium carbonate anhydrous, sodium hydroxide, sodium nitrite, and trichloroAcetic acid were purchased from Chempur, Poland. Neocuproine was delivered by J&K Scientific, Germany. Folin–Ciocalteu reagent, acetonitrile, iron(II) sulfate heptahydrate, gallic acid were supplied by Merck, Germany, whereas rutin trihydrate by Roth, Germany. ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), caffeine were purchased from Sigma-Aldrich, USA. All the chemicals were of analytical grade.
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2

Antioxidant Potential of Medicinal Plants

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The following amino acids were used for the synthesis: l-threonine (Thr, >99 wt%, pure), l-methionine (Met, >99 wt%, pure), l-arginine (Arg, >99 wt%, pure), l-isoleucine (Ile, >99 wt%, pure), purchased from Roth, and triethanolamine (TEA) (>99 wt%, pure) from Acros Organics. DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tris(2-pyridyl)-s-triazine) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) were purchased from Sigma-Aldrich (USA). Folin–Ciocalteu phenol reagent, iron(ii) sulfate heptahydrate and gallic acid were obtained from Merck (Germany), whereas ethanol, 99.5% acetic acid, sodium acetate anhydrous, 36% hydrochloric acid, iron(iii) chloride hexahydrate, copper(ii) chloride dihydrate, and sodium carbonate anhydrous were obtained from Chempur (Poland). Neocuproine was delivered by J&K Scientific (Germany).
The research plant material consisted of dry and cut L. clavatum herbs (Dary Natury, Poland), C. islandica (Kawon, Poland) and D. fullonum leaves (NatVita, Poland).
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Antioxidant Capacity Assays Protocol

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DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6--tris(2-pyridyl)-s-triazine), ABTS (2,2′-azino-bis(3--ethylbenzothiazoline-6-sulfonic acid) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxy-lic acid) were purchased from Sigma-Aldrich, USA; gallic acid, Folin-Ciocalteu reagent and iron(II) sulfate heptahydrate from Merck, Germany; rutin trihydrate from Roth, Germany; ferrozine from Serva Electrophoresis, Germany; neocuproine from J&K Scientific, Germany. Methanol, potassium persulfate, 36% hydrochloric acid, iron(III) chloride hexahydrate, 99.5% acetic acid, sodium acetate anhydrous, sodium carbonate anhydrous, copper(II) chloride dihydrate, iron(II) chloride tetrahydrate, potassium chloride, sodium nitrite, aluminum chloride hexahydrate and sodium hydroxide were obtained from Chempur, Poland; ethanol from Linegal Chemicals, Poland. All reagents were of analytical grade.
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4

Antioxidant Capacity Assays Protocol

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DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6--tris(2-pyridyl)-s-triazine), ABTS (2,2′-azino-bis(3--ethylbenzothiazoline-6-sulfonic acid) and Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxy-lic acid) were purchased from Sigma-Aldrich, USA; gallic acid, Folin-Ciocalteu reagent and iron(II) sulfate heptahydrate from Merck, Germany; rutin trihydrate from Roth, Germany; ferrozine from Serva Electrophoresis, Germany; neocuproine from J&K Scientific, Germany. Methanol, potassium persulfate, 36% hydrochloric acid, iron(III) chloride hexahydrate, 99.5% acetic acid, sodium acetate anhydrous, sodium carbonate anhydrous, copper(II) chloride dihydrate, iron(II) chloride tetrahydrate, potassium chloride, sodium nitrite, aluminum chloride hexahydrate and sodium hydroxide were obtained from Chempur, Poland; ethanol from Linegal Chemicals, Poland. All reagents were of analytical grade.
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