Hdac2 antibody

For CSE and HDAC2 immunohistochemical analysis of human pulmonary tissue, specimens were fixed, embedded in paraffin, cut into sections (4–6 μm), and stained with haematoxylin, as reported previously(7). The sections were incubated with mouse anti-human CSE antibody (1:25; Abnova) or HDAC2 antibody (1:50; Cell Signaling Technology) for 24 h at 4 °C. Anti-goat secondary antibody (1:100; ZSGB-BIO) conjugated with DAB was used for detection. Non-immune IgG isotype was used as a negative control.

All immunoblotting analyses were performed following SDS PAGE using standard methods. Antibodies used for immunoblotting were HIF1α antibody (Abcam, CAT# ab51608, dilution 1:1000), HIF1α antibody (Cell Signaling Technology, CAT# 79233, dilution 1:1000), EZH2 antibody (Cell Signaling Technology, CAT# 5246, dilution 1:1000), SUZ12 antibody (Cell Signaling Technology, CAT# 3737, dilution 1:1000), EED antibody (Millipore, CAT# 09-744, dilution 1:1000), HDAC1 antibody (Cell Signaling Technology, CAT# 34589, dilution 1:1000), HDAC2 antibody (Cell Signaling Technology, CAT# 57156, dilution 1:1000), phosphorylated-STAT1 antibody (Cell Signaling Technology, CAT# 9167, dilution 1:1000), STAT1 antibody (Cell Signaling Technology, CAT# 9176, dilution 1:1000), PD-L1 antibody (Cell Signaling Technology, CAT# 3684, dilution 1:1000), IRF1 antibody (Abcam, CAT# ab26109, dilution 1:1000), H3K27me3 antibody (Cell Signaling Technology, CAT# 9733, dilution 1:1000), H3K27ac antibody (Abcam, CAT# 4729, dilution 1:1000), and GAPDH antibody (Cell Signaling Technology, CAT# 2118, dilution 1:1000).