Anti ets1 antibody

Manufactured by Abcam

Sourced in United States

Anti-ets1 antibody is a laboratory reagent used for the detection and analysis of the ets1 protein, which is a transcription factor involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to identify and quantify the presence of ets1 in biological samples.

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Lab products found in correlation

Anti β actin antibody, by Merck Group (2 mentions) Simplechip plus sonication chromatin ip kit, by Cell Signaling Technology (1 mentions) Microson ultrasonic cell disruptor xl, by Bioventus (1 mentions) Ecl kit, by Merck Group (1 mentions) Bca assay, by Bio-Rad (1 mentions) Magna rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Control igg, by Abcam (1 mentions) Anti vimentin antibody, by Cell Signaling Technology (1 mentions) Anti foxm1 antibody, by Santa Cruz Biotechnology (1 mentions) Anti e cadherin antibody, by BD (1 mentions) Anti n cadherin antibody, by Cell Signaling Technology (1 mentions) Anti erk1 2 antibody, by Abcam (1 mentions) Anti hif 1α antibody, by Novus Biologicals (1 mentions) Anti akt antibody, by Cell Signaling Technology (1 mentions) Anti perk1 2 antibody, by Abcam (1 mentions) Anti snai1 antibody, by Cell Signaling Technology (1 mentions) Anti igf1r antibody, by Abcam (1 mentions)

4 protocols using anti ets1 antibody

ChIP Assay for PTP1B Gene

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ChIP assay was carried out using a SimpleChIP Plus Sonication Chromatin IP kit (Cell Signaling Technology, Beverly, USA) according to the manufacturer’s protocol. Briefly, cells were fixed with 1% formaldehyde and chromatin was sheared with a Microson XL ultrasonic cell disruptor (Misonix, Farmingdale, USA). Then, 10 μl of solution was used as the input. The surplus samples were incubated with anti-ets1 antibody (Abcam) or IgG for 10 h at 4°C. After the immunoprecipitants bound to protein G magnetic beads, the beads were washed and incubated at 65°C for 2 h. After purification, the sequence of DNA fragments was detected by PCR analysis. The primer sequences for
PTP1B are listed below: forward 5′-CATTATTCAACACACTTCCCA-3′, and reverse 5′-GGACACTTGTGCTATTTTGAG-3′.

Jiang L., Liang J., Wang T., Meng F, & Duan W. (2022). ETS proto-oncogene 1 modulates PTP1B expression to participate in high glucose-mediated endothelial inflammation: Ets1 modulates PTP1B expression to participate in inflammation. Acta Biochimica et Biophysica Sinica, 54(4), 565-573.

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HUVEC Protein Expression Analysis

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After treatment with DHA, HUVECs were lysed in RIPA lysis buffer with 0.1% SDS, 1 mg/ml leupeptin and 1 mM PMSF on ice. Proteins were quantified by BCA assay (Bio‑Rad, Hercules, CA, USA). The protein samples were loaded and separated on a 8% SDS‑polyacrylamide gel, then electroblotted onto the PVDF membranes. The membranes were blocked 1 h in 5% skim milk in TBS-T (TBS containing 0.05% Tween-20), and then incubated with the primary antibody at 4˚C overnight. The primary antibodies include anti-VEGFR1 antibody (Abcam, Cambridge, MA, USA), anti-ETS-1 antibody (Abcam) and anti-β-actin antibody (Sigma-Aldrich). The membranes were washed in TBS-T, and incubated with a HRP-linked goat anti-rabbit secondary antibody (Proteintech, Chicago, IL, USA) for 2 h at room temperature. The protein bands were visualized with an ECL kit (Millipore, Billerica, MA, USA).

Niu N., Yu C., Li L., Liu Q., Zhang W., Liang K., Zhu Y., Li J., Zhou X., Tang J, & Liu J. (2018). Dihydroartemisinin enhances VEGFR1 expression through up-regulation of ETS-1 transcription factor. Journal of Cancer, 9(18), 3366-3372.

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ETS1 RNA Immunoprecipitation Protocol

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RIP was performed using Magna RNA‐binding protein immunoprecipitation kit (Millipore Corp., Bedford, MA, USA). The cells were lysed in complete RNA lysis buffer and incubated in RIP immunoprecipitation buffer containing magnetic beads conjugated with anti‐ETS1 antibody (diluted for 1:200, Abcam, Cambridge, USA) or IgG control (diluted for 1:100, Abcam). The immunoprecipitated RNAs were then isolated by Proteinase K and subjected to subsequent qRT‐PCR detection.

Song R., Lei S., Yang S, & Wu S. (2021). LncRNA PAXIP1‐AS1 fosters the pathogenesis of pulmonary arterial hypertension via ETS1/WIPF1/RhoA axis. Journal of Cellular and Molecular Medicine, 25(15), 7321-7334.

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Comprehensive Western Blot Analysis

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Western blot analysis was performed using standard procedures. The following primary antibodies were used in the experiments: anti-FOXM1 antibody (Santa Cruz), anti-pAKT antibody (Peprotech, USA), anti-AKT antibody (Cell Signaling Technology, Beverly, MA, USA), anti-pGSK3β antibody (Proteintech) at 1:1000, anti-Snai1 antibody (Cell Signaling Technology), anti-pIGF1R antibody (Abcam, Cambridge, UK), anti-IGF1R antibody (Abcam, Cambridge, UK), anti-E-cadherin antibody (BD Biosciences, USA), anti-Vimentin antibody (Cell Signaling Technology), anti-N-cadherin antibody (Cell Signaling Technology), anti-pERK1/2 antibody (Abcam), anti-ERK1/2 antibody (Abcam), anti-HIF1α antibody (Novus Biologicals, USA), anti-ETS1 antibody (Abcam) and anti-β-actin antibody (Sigma, St. Louis, MO,USA).

Xing S., Tian Z., Zheng W., Yang W., Du N., Gu Y., Yin J., Liu H., Jia X., Huang D., Liu W, & Deng M. (2021). Hypoxia downregulated miR-4521 suppresses gastric carcinoma progression through regulation of IGF2 and FOXM1. Molecular Cancer, 20, 9.

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