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G8200

Manufactured by Promega
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The G8200 is a high-performance microplate reader designed for a variety of life science applications. It provides accurate and sensitive detection of fluorescent, luminescent, and absorbance-based assays. The G8200 features a flexible optical system, allowing for efficient data acquisition and analysis.

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Lab products found in correlation

G8210, by Promega (3 mentions) G8090, by Promega (2 mentions) Caspase glo 8 andcaspase glo 9 assay systems, by Promega (2 mentions) Infinite m200 pro, by Tecan (2 mentions) Zietd, by R&D Systems (1 mentions) Incucyte s3, by Sartorius (1 mentions) Incucyte 2021a software, by Sartorius (1 mentions) Incucyte annexin 5 dye for apoptosis, by Sartorius (1 mentions) Spectramax id3, by Molecular Devices (1 mentions) 96 well plate, by BD (1 mentions) Xtt reagent, by Merck Group (1 mentions)

5 protocols using g8200

1

Caspase-Mediated Apoptosis Quantification

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Promega’s caspase kits (G8090, G8200 and G8210) were used to run the assay and the methods follow the protocol provided in the kits.
The cells were seeded at a density of 10,000 cells per well in a white-walled 96-well luminometer plate and treated with IC50 value of PU for 72 h. Before starting the assay, Caspase-Glo Reagent was prepared by mixing Caspase Glo substrate with Caspase Glo Buffer provided in the kit. The plate was removed from the incubator and it was allowed to equilibrate to room temperature. Then, 100 µL of Caspase Glo reagent was carefully added to each well and the plate was sealed with a lid. The contents were mixed on a plate shaker 300–500 rpm for 30 s. The contents were incubated for 30 min at room temperature. The luminescence of each sample was measured using a spectrophotometer.
Ganesan T., Sinniah A., Chik Z, & Alshawsh M.A. (2020). Punicalagin Regulates Apoptosis-Autophagy Switch via Modulation of Annexin A1 in Colorectal Cancer. Nutrients, 12(8), 2430.
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2

Caspase Activity Quantification Protocol

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Caspase-8/9 activity was measured using Caspase-Glo® 8 and
Caspase-Glo® 9 Assay Systems (Promega, G8200 and G8210). Generally,
2.5× 104 macrophages were plated inside 96 well plates and
treated as described inside the figure legend. 100μl Reagent was added to
each well including blanks and mixed with contents of wells using a plate shaker
at 300–500rpm for 0.5–2 min. Incubate at room temperature for 30
min. Luminescence was recorded via a microplate reader (Tecan Group Ltd,
infinite M200 PRO).
Zhang X., Sergin I., Evans T.D., Jeong S.J., Rodriguez-Velez A., Kapoor D., Chen S., Song E., Holloway K.B., Crowley J.R., Epelman S., Weihl C.C., Diwan A., Fan D., Mittendorfer B., Stitziel N.O., Schilling J.D., Lodhi I.J, & Razani B. (2020). High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy. Nature metabolism, 2(1), 110-125.
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3

Caspase Activity Quantification Protocol

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Caspase-8/9 activity was measured using Caspase-Glo® 8 and
Caspase-Glo® 9 Assay Systems (Promega, G8200 and G8210). Generally,
2.5× 104 macrophages were plated inside 96 well plates and
treated as described inside the figure legend. 100μl Reagent was added to
each well including blanks and mixed with contents of wells using a plate shaker
at 300–500rpm for 0.5–2 min. Incubate at room temperature for 30
min. Luminescence was recorded via a microplate reader (Tecan Group Ltd,
infinite M200 PRO).
Zhang X., Sergin I., Evans T.D., Jeong S.J., Rodriguez-Velez A., Kapoor D., Chen S., Song E., Holloway K.B., Crowley J.R., Epelman S., Weihl C.C., Diwan A., Fan D., Mittendorfer B., Stitziel N.O., Schilling J.D., Lodhi I.J, & Razani B. (2020). High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy. Nature metabolism, 2(1), 110-125.
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4

Caspase Activity Assay in Breast Cancer

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Caspase-3/7 and Caspase-8 activity was assessed in breast cancer lines by incubating Caspase-Glo 3/7 (Promega, G8090) and −8 (Promega, G8200) reagent with cells for one hour in a 96-well plate and measuring luminescence with a plate reader (Molecular Devices). SUM159 and LM2 cells were treated with 50nM H3B-8800 for 36 hours or with 200nM SD6 and 1ug/mL ZVAD, ZIETD, Z-AEVD, or Z-LEHD (R&D Systems) as annotated. MYC-ER HME1 cells were treated with 10nM 4-OHT and 20nM H3B-8800. 2208L, PyMT-M, AT3, and 2208L cells were treated with 50nM H3B-8800. Luminescence was normalized to cell number determined by Hoechst 33342 staining of a duplicate plate, followed by nuclei counting using the Celigo Imaging Cell Cytometer (Brooks).
Bowling E.A., Wang J.H., Gong F., Wu W., Neill N.J., Kim I.S., Tyagi S., Orellana M., Kurley S.J., Dominguez-Vidaña R., Chung H.C., Hsu T.Y., Dubrulle J., Saltzman A.B., Li H., Meena J.K., Canlas G.M., Chamakuri S., Singh S., Simon L.M., Olson C.M., Dobrolecki L.E., Lewis M.T., Zhang B., Golding I., Rosen J.M., Young D.W., Malovannaya A., Stossi F., Miles G., Ellis M.J., Yu L., Buonamici S., Lin C.Y., Karlin K.L., Zhang X.H, & Westbrook T.F. (2021). Spliceosome-Targeted Therapies Trigger an Antiviral Immune Response in Triple-Negative Breast Cancer. Cell, 184(2), 384-403.e21.
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5

Multimodal Apoptosis Assessment Assay

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Caspase-Glo 8 a(Promega G8200), Caspase-Glo 3/7 assays (Promega G8090) and XTT cell viability-assay (Sigma-Aldrich 11465015001) were performed according to manufacturers instructions. In brief, Caspase 8 and caspase 3/7 activity were measured in in 96-well plates (Falcon) according to manufacturer’s recommendations. Luminescence was measured utilizing a Spectramax iD3 multi-mode microplate reader (molecular devices). Cell viability was measured colorimetrically utilizing the XTT reagent (Sigma-Aldrich, 11465015001) following manufacturer’s instructions with absorbance measured in a Spectramax iD3 multi-mode microplate reader (Molecular Devices). Apoptosis was assesed utilizing Incucyte Annexin V Dye for Apoptosis (Sartorius, 4641) imaged using an Incucyte S3, and images analyzed utilizing the Incucyte 2021A software (Sartorius).
Carracedo M., Ericson E., Ågren R., Forslöw A., Madeyski-Bengtson K., Svensson A., Riddle R., Christoffersson J., González-King Garibotti H., Lazovic B., Hicks R., Buvall L., Fornoni A., Greasley P.J, & Lal M. (2023). APOL1 promotes endothelial cell activation beyond the glomerulus. iScience, 26(6), 106830.
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