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6 protocols using malic acid

1

Amino Acid and Organic Acid Analysis

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Durashell AA analysis kit contained internal standard and 17 kinds of amino acid standard, which was purchased from Agela Technologies (Tianjin, China). Oxalic acid, tartaric acid, lactic acid, citric acid, succinic acid, hydrochloric acid (HCl), disodium hydrogen phosphate dodecahydrate (Na2HPO4•12H2O), potassium dihydrogen phosphate (KH2PO4), phosphoric acid (H3PO4) (all AR grade), and malic acid (BR grade) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Guanosine 5′‐monophosphate (5′‐GMP) disodium salt hydrate, 5′‐IMP, 5′‐AMP, and cytidine 5′‐monophosphate (5′‐CMP) were purchased from Sigma‐Aldrich (St. Louis, Mo., U.S.A.). Methanol, sulfosalicylic acid, and acetonitrile (ACN) (all HPLC‐grade) were purchased from Fisher Scientific (Shanghai, China). The ultrapure water was purchased from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China). Buffer salt I (0.05 mol/L KH2PO4) was prepared by dissolving 6.80 g KH2PO4 in 1 L water. Buffer salt II (0.01 mol/L KH2PO4) was prepared by dissolving 1.36 g KH2PO4 in 1 L water and then adjusted to pH 2.8 by 1 mol/L H3PO4.
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2

Choline and Malic Acid Synthesis

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Choline chloride was purchased from Yuanye Bio-Technology Co., Ltd. Malic acid, HCl, and NaOH were purchased from Sinopharm Chemical Reagent Co., Ltd.
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3

Analytical Procedures for L-cysteine and PAT

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Standard L-cysteine (purity ≥ 99.0%) and PAT (purity ≥ 98.0%) were purchased from the Aladdin Industrial Corporation (Shanghai, China) and the Sangon Biotech (Shanghai, China) Co., Ltd., respectively. Acetonitrile and formic acid were HPLC grade, and were obtained from the Oceanpak Alexative Chemical Ltd., (Gothenburg, Sweden) and the Anpel Laboratory Technologies Inc. (Shanghai, China), respectively. Malic acid and ethyl acetate were analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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4

Chromium-based Compound Synthesis

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Chromium trichloride, malic acid, and chromium picolinate were bought in Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). The chromium malate was synthesized in our research group. Nitric and perchloric acid were purchased from Sinopharm Chemical Reagent Co., Ltd. Ultrapure water was used in this experiment.
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5

Analytical Characterization of Elegant Spices

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The properties of the elegant spices are summarized in Table 1. L‐(+)‐Tartaric acid, formic acid, lactic acid, acetic acid, citric acid, fumaric acid, succinic acid, L‐(+)‐ascorbic acid, propionic acid, potassium dihydrogen phosphate dodecahydrate phosphate (all AR grade), and malic acid (BR grade) were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China). Potassium dihydrogen phosphate (KH2PO4), phosphoric acid (H3PO4), hydrochloric acid (HCl), and disodium hydrogen phosphate dodecahydrate (Na2HPO4•12H2O) (all AR grade) were purchased from Sinopharm Chemical Reagent Company (Shanghai, China). Inosine 5′‐monophosphate (5′‐IMP), adenosine 5′‐monophosphate (5′‐AMP), guanosine 5′‐monophosphate (5′‐GMP), and cytidine 5′‐monophosphate (5′‐CMP) were purchased from Sigma‐Aldrich (St. Louis). Durashell AA analytical reagents, including an internal standard solution, were purchased from Tianjin Bona Agel Technology Co., Ltd. Methanol, trifluoroacetic acid (TFA), and acetonitrile (ACN) (all HPLC grade) were purchased from Fisher Scientific (Shanghai, China). Ultrapure water was purchased from Hangzhou Wahaha Group Co., Ltd. Sulfosalicylic acid (AR grade) was obtained from Biochemical Technology Co., Ltd.
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6

Cotton Fabric Chemical Modification

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Cotton fabric used in this paper is plain weave (40 s × 40 s, 133 × 72, weighing 108.6 g/m 2 ) desized, scoured, bleached and does not subject to any finishing processes. It was supplied by Youngor Group Co., Ltd. Acryloyl chloride (99%, J&K Scientific Co., Ltd.), maleic acid (99%, Sinopharm Chemical Reagent Co., Ltd.), sodium hypophosphite (99%, Sinopharm Chemical Reagent Co., Ltd.), malic acid (99%, Sinopharm Chemical Reagent Co., Ltd.), potassium bromide (99%, Sinopharm Chemical Reagent Co., Ltd.), soap flake (specially made for testing of textiles, Shanghai Soap Factory) were used as commercial products without any further purification.
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