For Bax oligomerization, the cells were incubated with conjugation buffer containing 10 mM EDTA, and 0.2 mM Bismaleimide (Thermo Scientific, Hudson, NH, USA) was added at room temperature for 1 h. Lysates were extracted by lysis buffer for Western blot analysis.
Bismaleimide
Manufactured by Thermo Fisher Scientific
Sourced in United States
Bismaleimide is a chemical compound used in various laboratory applications. It serves as a crosslinking agent, facilitating the formation of covalent bonds between molecules. The core function of bismaleimide is to enable the linking of compounds, such as proteins or other biomolecules, for the purpose of analysis, purification, or modification.
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Lab products found in correlation
3 protocols using bismaleimide
1
Bax Oligomerization via Bismaleimide
2
Bax Oligomerization Quantification by Flow Cytometry
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After fixing using 4% paraformaldehyde for 30 minutes, and cells were incubated for 1 hour at 4°C with the Bax-NT antibody (1:500; BD Biosciences) in PBS/1% FCS (Gibco, Carlsbad, CA, USA) + 0.1% saponine (Sigma Chemical Co.). After incubation with the secondary antibody, washing and re-suspension in PBS/1% FCS, and cells were measured by flow cytometry. For Bax oligomerization, the cells were suspended by conjugation buffer with 10 mM EDTA containing 0.2 mM bismaleimide (Thermo Scientific, Hudson, NH, USA) at room temperature for 1 hour and then extracted by lysis buffer for Western blot analysis.
3
STAT3 Dimerization Assay Protocol
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For STAT3 dimerization, the cells were suspended in conjugation buffer with 10 mM EDTA. The cell lysates were incubated with 0.2 mM bismaleimide (Thermo scientific, Rockford, IL, USA) at RT for 1 h, and then extracted with lysis buffer for Western blotting.
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