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Anti cul1

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Anti-Cul1 is a primary antibody that recognizes the Cullin 1 protein. Cullin 1 is a core component of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex, which is involved in the regulation of cell cycle progression and other cellular processes through the targeted ubiquitination and degradation of specific substrate proteins.

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Lab products found in correlation

Anti skp1, by Cell Signaling Technology (1 mentions) Ab68455, by Abcam (1 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Anti cdc2, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti cyclin b1, by Cell Signaling Technology (1 mentions) Anti p cdc2, by Cell Signaling Technology (1 mentions) Goat anti mouse igg hrp antibody, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti flag m2, by Merck Group (1 mentions) Anti tubulin, by Cell Signaling Technology (1 mentions) Anti nedd8, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti pakt s473, by Cell Signaling Technology (1 mentions) Anti irs1, by Cell Signaling Technology (1 mentions) Anti uchl1, by Cell Signaling Technology (1 mentions) Anti flag, by Cell Signaling Technology (1 mentions)

3 protocols using anti cul1

1

Immunoprecipitation and Western Blot

Cited 1 time
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IB assay was performed as previously described [27 (link)]. As to immunoprecipitation, cells were lysed, incubated with 2 μg corresponding antibodies overnight and incubated with protein A/G agarose for 4 h. The immunocomplexes were then resuspended with 2 × sample loading buffer and boiled for 5 min for SDS-PAGE and IB analysis. The primary antibodies used were: anti-Skp2 (ab68455, Abcam); anti-Skp1 (#12248, Cell Signaling Technology); anti-Cul1 (#4995, Cell Signaling Technology).
Ma X., Zhao J., Yang F., Liu H, & Qi W. (2017). Ubiquitin conjugating enzyme E2 L3 promoted tumor growth of NSCLC through accelerating p27kip1 ubiquitination and degradation. Oncotarget, 8(48), 84193-84203.
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2

Comprehensive Protein Immunodetection

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The antibodies used in this study were as follows: anti-Cyclin B1, anti-pCdc2 (Tyr15), anti-Cdc2, anti-ubiquitin, anti-E-cadherin, anti-NIPA, anti-Cul1, goat anti-rabbit IgG-HRP and goat anti-mouse IgG HRP antibody (Cell Signaling Technology, Beverly, MA); anti-α-tubulin and anti-Skp1 (Santa Cruz Biotechnology, Santa Cruz, CA); human anti-centromere proteins serum (Antibodies Inc., Davis, CA); anti-Flag M2, and anti-β-Actin (Sigma).
Liu Y.Q., Wang X.L., Cheng X., Lu Y.Z., Wang G.Z., Li X.C., Zhang J., Wen Z.S., Huang Z.L., Gao Q.L., Yang L.N., Cheng Y.X., Tao S.C., Liu J, & Zhou G.B. (2015). Skp1 in lung cancer: clinical significance and therapeutic efficacy of its small molecule inhibitors. Oncotarget, 6(33), 34953-34967.
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3

Drosophila Akt Signaling Pathway Antibodies

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Anti-pAkt (S505) for Drosophila (4054S, Cell Signaling), anti-DILP2 (provided by Dr. K. Yu)20 (link), anti-pAkt (S473) (4060S, Cell Signaling), anti-Akt (9272S, Cell Signaling), anti-Flag (2368S, Cell Signaling), anti-Flag (M185-3L, MBL), anti-UCHL1 (13179S, Cell Signaling), anti-Tubulin (DSHB), anti-HA (3724S, Cell Signaling), anti-IRS1 (2390S, Cell Signaling), anti-Myc (M192-3, MBL), anti-CUL1 (4995S, Cell Signaling), anti-NEDD8 (2745, Cell Signaling), anti-mouse HRP (115-035-146, Jackson), anti-rabbit HRP (111-035-144, Jackson), anti-rabbit TRITC (111-296-144, Jackson), and anti-Neurofilament-H (NF-H) (AB5539, Sigma) antibodies were used in this study.
Lee D., Yoon E., Ham S.J., Lee K., Jang H., Woo D., Lee D.H., Kim S., Choi S, & Chung J. (2024). Diabetic sensory neuropathy and insulin resistance are induced by loss of UCHL1 in Drosophila. Nature Communications, 15, 468.
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