Chromogenic substrate

Manufactured by Merck Group

Sourced in United Kingdom, United States

Chromogenic substrate is a type of laboratory reagent used in various analytical techniques. It is designed to produce a visible color change upon a specific biochemical reaction, allowing for the detection and quantification of target analytes. The core function of a chromogenic substrate is to provide a simple and sensitive means of monitoring and measuring the activity of enzymes or other biomolecules in a sample.

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Lab products found in correlation

Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) N p tosyl gly pro arg p nitroanilide acetate, by Merck Group (1 mentions) Bc thrombin, by Siemens (1 mentions) Tygon poly vinyl chloride pvc tubing, by Thermo Fisher Scientific (1 mentions) Carageenan, by Merck Group (1 mentions) Thrombin, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Chloroform, by Thermo Fisher Scientific (1 mentions) Phospholipid 90g, by Lipoid (1 mentions) Cholesterol, by Alpha Chemika (1 mentions) Span 80, by SD Fine Chemicals (1 mentions) Span 60, by SD Fine Chemicals (1 mentions)

Spelling variants of this product

ECL chromogenic substrate (9 citations) Substrates (2 citations) UPA-specific chromogenic substrate (2 citations) Alkaline phosphatase chromogenic substrate (2 citations)

3 protocols using chromogenic substrate

Chromogenic Assay for TPA Activity

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The activity of TPA in P. pastoris culture supernatants was assessed with a chromogenic substrate (Sigma, Dorset, UK). Reactions consisting of 219 µL 50 mM Tris-HCl pH 8, 25 µL 2 mM substrate (solubilised in dH₂O) and 6.25 µL culture supernatant were assembled in a 96 well plate and the absorbance measured at 405 nm. The plate was sealed and incubated for 16 hours at 37 °C after which the final absorbance reading was measured.

Royle K.E, & Polizzi K. (2017). A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris. Scientific Reports, 7, 15817.

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Argatroban Polymer Solution Synthesis

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Materials for synthesis of the argatroban polymer solution were obtained from the following: CarboSil^® polymer from DSM Biomedical (Berkeley, CA); anhydrous hexane from Fisher Scientific (Fair Lawn, NJ); anhydrous tetrahydrofuran (THF), anhydrous hexamethylene diisocyanate (HMDI), argatroban monohydrate ((2F,4R)-1-[(2S)-5-(diaminomethylideneamino)-2-[[(3R)-3-methyl-1,2,3,4-tetrahydroquinolin-8-yl]sulfonylamino]pentanoyl]-4-methyl-piperidine-2-carboxylic acid) all from Sigma-Aldrich (St. Louis, MO). Hanks Balanced Salt Solution (HBSS) with calcium and magnesium was purchased from Gibco^® (Grand Island, NY). Materials for the chromogenic antithrombin assay were obtained from the following: chromogenic substrate (N-p-tosyl-Gly-Pro-Arg-p-nitroanilide acetate) from Sigma-Aldrich (St. Louis, MO); BC thrombin from Siemens (Newark, DE). ECC circuits were constructed from the following: Tygon^® poly(vinyl chloride) (PVC) tubing from Fisher Healthcare (Houston, TX); polyurethane angiocatheters, 16-gauge and 14-gauge, from Kendall Monoject Tyco Healthcare (Mansfield, MA).

Yu J., Brisbois E., Handa H., Annich G., Meyerhoff M., Bartlett R, & Major T. (2016). The immobilization of a direct thrombin inhibitor to a polyurethane as a nonthrombogenic surface coating for extracorporeal circulation. Journal of materials chemistry. B, Materials for biology and medicine, 4(13), 2264-2272.

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Synthesis and Characterization of Aspirin Nanoformulations

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Isopropyl isothiocyanate, was purchased from Sigma Aldrich, St. Louis, MO, USA. Span 20, span 40, span 60, and span 80 were purchased from SD fine chemicals, Mumbai, India. Phospholipid 90G was received as gift sample from Lipoid GmbH, Ludwigshafen, Germany. Acetylsalicylic acid (Aspirin) was purchased from CDH Fine chemicals, New Delhi, India. The other materials, thrombin, carageenan, and chromogenic substrate, were procured from Sigma Aldrich, St. Louis, MO, USA. Cholesterol was purchased from Alpha Chemika, (Mumbai, India). Methanol and chloroform was purchased from Fisher Scientific, Loughborough, UK.

Kala C., Asif M., Gilani S.J., Imam S.S., Khan N.A., Taleuzzaman M., Zafar A., Ahmed M.M., Alshehri S, & Ghoneim M.M. (2022). Formulation of Isopropyl Isothiocyanate Loaded Nano Vesicles Delivery Systems: In Vitro Characterization and In Vivo Assessment. Molecules, 27(9), 2876.

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