N n dimethylacrylamide dma

Acryloyl chloride (>97%, Sigma-Aldrich (Munich, Germany), contained 400 ppm phenothiazine as stabilizer, stored at 4 °C), anhydrous dichloromethane (DCM) (99.9%, extra dry, Acros Organics (Geel, Belgium)), 4,4′-azobis(4-cyanovaleric acid) (ACVA) (>98%, Sigma-Aldrich, stored at 4 °C), 2-(dodecylthiocarbonothioylthio)propionic acid (DTPA) (97%, Sigma-Aldrich, stored at 4 °C) and N,N-dimethylformamide (DMF) (>99.5%, VWR Chemicals (Darmstadt, Germany)) were used as received. Pyrrolidine (>99%, Acros Organics) and piperidine (99%, Acros Organics) were stored at 4 °C over molecular sieves (mesh size = 4 Å). Styrene (99%, Grüssing (Filsum, Germany), stored at 4 °C) and N,N-dimethylacrylamide (DMA) (99%, Sigma-Aldrich, stored at 4 °C) were freshly percolated through a column of basic alumina (>98%, Brockmann I, Sigma-Aldrich) prior to use to remove the inhibitor methyl ether hydroquinone. DOX (99%, Grüssing) was stored over KOH pellets (>85%, Merck (Darmstadt, Germany)) and freshly percolated through a basic alumina column prior to use to remove peroxides. Ultrapure water (MilliQ quality, resistivity >18.2 MΩ·cm⁻¹) was obtained from a Millipore (Darmstadt, Germany) MilliQ water purification system. All other chemicals were used as received in at least analytical grade.

Acrylic acid (AAc) stabilized with hydroquinone with a purity of 99.5% extra pure (CAS 79-10-7) was supplied by Acros Organics (Geel, Belgium). N,N-dimethyl acrylamide (DMA, 99%), ammonium persulfate (98%, APS, CAS 7727-54-0), and N,N-methylene-bis-acrylamide (99%, CAS 110-26-9) were obtained from Sigma-Aldrich (Heidelberg, Germany). Furthermore, the gentamicin of clinical grades was obtained from Kazakhstan in the specification of 1 mL:40 units (Shymkent, Kazakhstan, 40 mg/mL). In addition, several types of bacterial culture were obtained from the laboratory.