For intracellular cytokine staining, T cells were activated for 3h with PMA and ionomycin in the presence of GolgiStop (BD Bioscience), followed by anti-IFNγ (4S.B3; Biolegend) or anti-IL-17 (eBio64Dec17; ebiosciences). Levels of IFNγ and IL-17 secreted by T cells stimulated for 4 days under Th1-, Th2- or Th17-polarizing conditions were measured using a Cytometric Bead Array (CBA) Flex Set system (BD Bioscience) and analyzed with FCAP Array Software (BD Bioscience). Cytokine levels in serum were determined by the University of Pittsburgh Cancer Institute Luminex Core Laboratory using MILLIPLEX NHP kits from Millipore (Billerica, MA), according to the manufacturer’s instructions.
Milliplex nhp kits
Manufactured by Merck Group
MILLIPLEX NHP kits are a multiplex assay platform developed by Merck Group for the simultaneous detection and quantification of multiple analytes in non-human primate (NHP) samples. The kits utilize Luminex technology to provide a rapid and sensitive method for analyzing multiple biomarkers in a single well, allowing researchers to obtain more data from limited sample volumes.
Automatically generated - may contain errors
2 protocols using milliplex nhp kits
1
Cytokine Profiling of Activated T Cells
2
Serum Cytokine Profiling with Luminex
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