H peo

Manufactured by Colorcon

Sourced in United Kingdom

The H-PEO is a laboratory equipment designed for the characterization and analysis of polyethylene oxide (PEO) samples. It provides accurate measurements of the molecular weight and molecular weight distribution of PEO polymers.

Automatically generated - may contain errors

Lab products found in correlation

L peo, by Merck Group (3 mentions) Kolliphor p407 poloxamer, by BASF (2 mentions) Rt4 d6p2t, by American Type Culture Collection (1 mentions) Trypsin edta solution, by Avantor (1 mentions) Trypan blue solution, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Chitosan, by Ametek (1 mentions) Nile red, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Sodium hydroxide (naoh), by Carlo Erba (1 mentions) Hydrochloric acid (hcl), by Carlo Erba (1 mentions) Cacl2, by Carlo Erba (1 mentions) Kolliphor p407, by BASF (1 mentions)

4 protocols using h peo

Electrospinning of Polymeric Solutions for Neuronal Cell Culture

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Deacetylated gellan gum (GG, Gelrite^®; Kelco Division of Merck & Co., Rahway, New Jersey, USA), spermidine trihydrochloride (SP; Sigma Aldrich, Milan, Italy), gelatin from porcine skin (GL, gel strength 90–110 g Bloom, Type A; Sigma Aldrich, Milan, Italy), poly(ethylene oxide) of high molecular weight (h-PEO, MW = 4000 kDa; Colorcon, Dartford, United Kingdom), poly(ethylene oxide) of low molecular weight (l-PEO, MW = 600 kDa; Sigma Aldrich, Milan, Italy) and Kolliphor P407 poloxamer (P407; Sigma Aldrich, Milan, Italy) were used for the preparation of the polymeric solutions to be electrospun.
For the experiments with Neuronal Schwann cells RT4-D6P2T (ATCC CRL-2768), the materials hereafter reported were used. Dulbecco’s Modified Eagle’s Medium (ATCC-30-2002) was purchased from LGC Standards S.r.L. (Milan, Italy), while inactivated Fetal Bovine Serum (FBS) from Biowest (Nuaillé, France). Phosphate Buffer Solution (PBS), antibiotic/antimycotic solution (100X; stabilized with 10,000 units penicillin, 10 mg streptomycin, and 25 g amphotericin B per mL) and trypsin–EDTA solution were purchased from VWR (Radnor, Pennsylvania, USA). Dimethyl sulfoxide (DMSO), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Trypan Blue solution were purchased from Sigma-Aldrich (Milan, Italy).

Vigani B., Valentino C., Sandri G., Caramella C.M., Ferrari F, & Rossi S. (2022). Spermidine Crosslinked Gellan Gum-Based “Hydrogel Nanofibers” as Potential Tool for the Treatment of Nervous Tissue Injuries: A Formulation Study. International Journal of Nanomedicine, 17, 3421-3439.

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Lipid Nanoparticle Formulation and Characterization

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For the preparation of lipid nanoparticles, the following materials were used: chitosan low molecular weight (LCS; MW = 50,000–190,000 Da; 20–300 cP, 1% w/w in 1% acetic acid, 25 °C, Brookfield; 75–85% deacetylated), chitosan medium molecular weight (MCS; MW = 190,000–310,000 Da; 200–800 cP, 1% w/w in 1% acetic acid, 25 °C, Brookfield; 75–85% deacetylated), chitosan high molecular weight (HCS; MW = 310,000–375,000 Da; 800–2000 cP, 1% w/w in 1% acetic acid, 25 °C, Brookfield; >75% deacetylated), poly(vinyl alcohol) (PVA; MW = 9000–10,000 Da; 80% hydrolyzed), and Nile Red (NR) were purchased from Sigma Aldrich (Milan, Italy), sodium stearate (Na-SA) was obtained from AAKON Polichimica Srl (Milan, Italy), sodium behenate (Na-BA) was obtained from Larodan, Research Grade Lipids (Monroe, MO, USA), and sodium hydroxide (NaOH 0.1M) and hydrochloric acid (HCl 1M) were obtained from Carlo Erba Reagents Srl (Milan, Italy).
All reagents used for cell cultures were purchased from Sigma Aldrich (Milan, Italy).
For the synthesis of O-carboxymethyl chitosan (O-CMCS), all the reactants and solvents were supplied by Sigma Aldrich (Milan, Italy).
For the preparation of electrospun nanofibers, the following materials were used: Kolliphor P407 poloxamer (P407, BASF SE, Ludwigshafen, Germany) and poly(ethylene oxide) high molecular weight (h-PEO, MW = 4000 kDa, Colorcon, Dartford, UK).

Vigani B., Valentino C., Sandri G., Listro R., Fagiani F., Collina S., Lanni C., Bonferoni M.C., Caramella C.M., Rossi S, & Ferrari F. (2021). A Composite Nanosystem as a Potential Tool for the Local Treatment of Glioblastoma: Chitosan-Coated Solid Lipid Nanoparticles Embedded in Electrospun Nanofibers. Polymers, 13(9), 1371.

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Polymeric Solutions for Nanofibrous Scaffolds

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For the preparation of polymeric solutions the following materials were used: Deacetylated gellan gum (GG, Gelrite^®, Kelco Division of Merck & Co., Rahway, NJ, USA), Kolliphor P407 poloxamer (P407, BASF SE, Ludwigshafen, Germany), poly (ethylene oxide) of high molecular weight (h-PEO, MW = 4000 kDa, Colorcon, Dartford, UK), poly (ethylene oxide) of low molecular weight (l-PEO, MW = 600 kDa, Sigma Aldrich, Milan, Italy) and glycine (Gly, Sigma Aldrich, Milan, Italy). GG-based NFs cross-linking was performed using anhydrous calcium chloride (CaCl₂) and absolute ethanol (EtOH; Carlo Erba Reagents S.r.l., Milan, Italy).
Reagents and solvents for RC-33 synthesis were purchased from Merck (Milan, Italy).

Vigani B., Valentino C., Cavalloro V., Catenacci L., Sorrenti M., Sandri G., Bonferoni M.C., Bozzi C., Collina S., Rossi S, & Ferrari F. (2021). Gellan-Based Composite System as a Potential Tool for the Treatment of Nervous Tissue Injuries: Cross-Linked Electrospun Nanofibers Embedded in a RC-33-Loaded Freeze-Dried Matrix. Pharmaceutics, 13(2), 164.

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Preparation of Alginate-PEO Polymer Blends

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Alginic acid sodium salt from brown algae (ALG, medium viscosity, Brookfield viscosity ≥ 2000 cps (2% in water at 25 °C), G/M ratio of 70:30, Sigma Aldrich, St. Louis, MO, USA), poly(ethylene oxide) of high molecular weight (h-PEO, MW = 4000 kDa, Colorcon, Dartford, United Kingdom), poly(ethylene oxide) of low molecular weight (l-PEO, MW = 600 kDa, Sigma Aldrich, St. Louis, MO, USA), Kolliphor P407 (P407, BASF SE, Ludwigshafen, Germany), and Triton X-100 (TX100, Fluka BioChemika, Buchs, Switzerland) were used for the preparation of ALG/PEO solutions.

Vigani B., Rossi S., Milanesi G., Bonferoni M.C., Sandri G., Bruni G, & Ferrari F. (2018). Electrospun Alginate Fibers: Mixing of Two Different Poly(ethylene oxide) Grades to Improve Fiber Functional Properties. Nanomaterials, 8(12), 971.

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