The largest database of trusted experimental protocols

Annexin 5 fitc ann

Manufactured by Thermo Fisher Scientific

Annexin-V/FITC (Ann) is a fluorescent-labeled protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis (programmed cell death). This product can be used to detect and quantify apoptotic cells in various applications.

Automatically generated - may contain errors

2 protocols using annexin 5 fitc ann

1

Apoptosis Assay in HCEnC-21T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCEnC-21T cells were seeded in a 6-well plate and cultured for 24 h. Cells were treated with/without MN (50 μM) or 4-OHE2 (10 μM), and incubated for 16 h in estrogen-free medium. Cells were then trypsinized, washed in cold PBS, and incubated in the dark with 3 μL of Annexin-V/FITC (Ann) (Thermo Fisher Scientific) and 2 μL of propidium iodide (PI, Sigma-Aldrich) in a total volume of 50 μL for 30 min at room temperature. Cells were re-suspended in 2 mL of Isoflow sheath fluid (Beckman Coulter, Fullerton, CA) and 20,000 live cells were acquired on a LSR II (BD Biosciences) with DIVA software (BD Biosciences) gated for PI and Annexin V with unstained cells as a negative control. Data were then analyzed with Summit 4.3 (Beckman Coulter, Fullerton, CA).
+ Open protocol
+ Expand
2

MN-Induced Apoptosis in NQO1 Knockout Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCEnC-21T control (NQO1+/+) and NQO1/ cells were seeded in a 6-well plate and cultured for 24 hours prior to treating with 50μM MN in low-glucose DMEM) and incubated for 5 hours. Cells were then trypsinized, washed in cold PBS, and incubated in the dark with 3μl of Annexin-V/FITC (Ann) (Thermo Fisher, Waltham, MA) and 2μl of PI (Sigma-Aldrich) in a total volume of 50μl for 30 minutes at room temperature. Cells were re-suspended in 2ml of Isoflow sheath fluid (Beckman Coulter, Fullerton, CA) and 20,000 live cells were acquired on a LSR II (BD Biosciences) with DIVA software (BD Biosciences) gated for PI and Annexin V with unstained cells as negative control. Data were then analyzed with Summit 4.3 (Beckman Coulter, Fullerton, CA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!