Single-cell suspensions from pLN were incubated with various combinations of fluorochrome-conjugated rat anti-mouse monoclonal antibodies specific for CD4 (L3T4), CD11b (M1/70), CD11c (HL3), MHC-II I-A/I-E (M5/114.15.2), Ly6G (1A8), Vβ11 (RR3-15) (BD Biosciences), CD326/EpCAM (G8.8), CD103 (2E7) (Biolegend), CD64 (X54-5/7) and CD4 (RM4-5) (eBiosciences) for 45 min at 4 °C in FACS buffer containing 0.5 mg/mL anti-mouse CD16/CD23 (2.4G2) (BD Biosciences). Flow cytometry was performed on an LSR-II with BD FACSDIVA software (BD Biosciences). The acquired data analyzed on FlowJo software (BD Biosciences).
Anti mouse cd16 cd23 2.4g2
Manufactured by BD
The Anti-mouse CD16/CD23 (2.4G2) is a laboratory reagent that can be used to detect the expression of CD16 and CD23 on mouse cells. It is a rat monoclonal antibody that binds to these cell surface receptors. The product can be used in various immunological techniques, such as flow cytometry, to study the distribution and function of these markers in mouse models.
Automatically generated - may contain errors
3 protocols using anti mouse cd16 cd23 2.4g2
1
Multiparametric Flow Cytometry Analysis
2
Multiparameter Flow Cytometry Immunophenotyping
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BMDCs or single-cell suspensions from pLNs were incubated with various combinations of fluorochrome-conjugated anti-mouse mAbs specific for CD11b (M1/70), CD11c (HL3), CD40 (HM40-3), MHC class II I-A/I-E (M5/114.15.2) (BD Biosciences), CD86 (GL-1), CD326/EpCAM (G8.8), and CD103 (2E7) (BioLegend) for 45 min at 4°C in FACS buffer containing 0.5 mg/ml anti-mouse CD16/CD23 (2.4G2) (BD Biosciences), except for CCR7 (4B12) and accompanying isotype control (rat IgG2a) (both from Miltenyi), where incubations were performed for 10 min at room temperature. Flow cytometry was performed on an LSR-II with FACSDiva software (BD Biosciences). Acquired data were analyzed with FlowJo software (BD Biosciences).
3
Multiparameter Flow Cytometry of Lymph Node Cells
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Single-cell suspensions from pLN were incubated with various combinations of fluorochrome-conjugated rat anti-mouse mAbs specific for CD4 (L3T4), CD11b (M1/70), CD11c (HL3), MHC-II I-A/I-E (M5/114.15.2), Ly-6G (1A8), Vβ11 (RR3-15) (BD Biosciences), CD326/EpCAM (G8.8), CD103 (2E7) (BioLegend), CD64 (X54-5/7), and CD4 (RM4-5) (eBiosciences) for 45 min at 4°C in FACS buffer containing 0.5 mg/ml anti-mouse CD16/CD23 (2.4G2) (BD Biosciences). Flow cytometry was performed on an LSR II with BD FACSDiva software (BD Biosciences). The acquired data were analyzed on FlowJo software (BD Biosciences).
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