HUVECs grown on eight‐well cell culture slides (Mat‐Tek) coated with Fibronectin (Corning; Sigma‐Aldrich) were washed with warmed PBS (37°C) twice, fixed in warm 4% paraformaldehyde (Thomas Scientific; 37°C) for 5 min and washed with PBS three times. The cells were permeabilized with 1% Triton X‐100 in PBS for 5 min and washed again with PBS three times. The cells were blocked with 1% bovine serum albumin (Sigma‐Aldrich) in PBS for 30 min. Anti‐acetylated‐tubulin (T6793; Sigma‐Aldrich; 1:1000), anti‐Arl13B (17711‐1‐AP; Proteintech; 1:500), anti‐IFT81 (11744‐1‐AP; Proteintech; 1:500), and anti‐BMPR‐II (612292; BD Biosciences; 1:500) antibodies were incubated overnight at 4°C, and then detected with Alexa Fluor 568 donkey anti‐mouse IgG (A10037; Invitrogen; 1:500), Alexa Fluor 568 goat anti‐rabbit IgG (A11011; Invitrogen; 1:200), Alexa Fluor 488 goat anti‐mouse IgG (A11001; Invitrogen; 1:200), and/or Alexa Fluor 647 goat anti‐rabbit IgG (A21244; Invitrogen; 1:200). 0.5 µg/ml DAPI (Sigma‐Aldrich) was used to stain the nuclei. The cells were mounted using Vectashield (Vector Labs) and imaged using fluorescence microscopy.
Anti ift81 11744 1 ap
Manufactured by Proteintech
Anti‐IFT81 (11744‐1‐AP) is a primary antibody produced in rabbit that targets the IFT81 protein. IFT81 is a component of the intraflagellar transport complex B, which is involved in the assembly and maintenance of cilia and flagella.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 568 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 568 donkey anti mouse igg, by Thermo Fisher Scientific (2 mentions)
Alexa fluor 647 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions)
Anti bmpr 2 612292, by BD (2 mentions)
Anti arl13b 17711 1 ap, by Proteintech (2 mentions)
Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Vectashield, by Vector Laboratories (2 mentions)
Fibronectin, by Corning (2 mentions)
Anti acetylated tubulin t6793, by Merck Group (1 mentions)
2 protocols using anti ift81 11744 1 ap
1
Immunofluorescence Staining of Primary Cilia
2
Immunofluorescence Staining of HUVECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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HUVECs grown on 8-well cell culture slides (Mat-Tek) coated with Fibronectin (Corning, Sigma-Aldrich)
were washed with warmed PBS (37 ℃) twice, fixed in warm 4% paraformaldehyde (Thomas Scientific, 37 ℃) for 5 min and washed with PBS 3 times. The cells were permeabilized with 1% Triton X-100 in PBS for 5 min and washed again with PBS 3 times. The cells were blocked with 1% bovine serum albumin (Sigma-Aldrich) in PBS for 30 min. Anti-acetylated-tubulin (T6793, Sigma-Aldrich, 1:1000), anti-Arl13B
(17711-1-AP, Proteintech, 1:500), anti-IFT81 (11744-1-AP, Proteintech, 1:500) and anti-BMPR-II (612292, BD Biosciences, 1:500) antibodies were incubated overnight at 4℃, and then detected with Alexa Fluor 568 donkey anti-mouse IgG (A10037, Invitrogen, 1:500), Alexa Fluor 568 goat anti-rabbit IgG (A11011, Invitrogen, 1:200), Alexa Fluor 488 goat anti-mouse IgG (A11001, Invitrogen, 1:200) and/or Alexa Fluor 647 goat anti-rabbit IgG (A21244, Invitrogen, 1:200). 0.5 µg/ml DAPI (Sigma-Aldrich) was used to stain the nuclei. The cells were mounted using Vectashield (Vector Labs) and imaged using fluorescence microscopy.
were washed with warmed PBS (37 ℃) twice, fixed in warm 4% paraformaldehyde (Thomas Scientific, 37 ℃) for 5 min and washed with PBS 3 times. The cells were permeabilized with 1% Triton X-100 in PBS for 5 min and washed again with PBS 3 times. The cells were blocked with 1% bovine serum albumin (Sigma-Aldrich) in PBS for 30 min. Anti-acetylated-tubulin (T6793, Sigma-Aldrich, 1:1000), anti-Arl13B
(17711-1-AP, Proteintech, 1:500), anti-IFT81 (11744-1-AP, Proteintech, 1:500) and anti-BMPR-II (612292, BD Biosciences, 1:500) antibodies were incubated overnight at 4℃, and then detected with Alexa Fluor 568 donkey anti-mouse IgG (A10037, Invitrogen, 1:500), Alexa Fluor 568 goat anti-rabbit IgG (A11011, Invitrogen, 1:200), Alexa Fluor 488 goat anti-mouse IgG (A11001, Invitrogen, 1:200) and/or Alexa Fluor 647 goat anti-rabbit IgG (A21244, Invitrogen, 1:200). 0.5 µg/ml DAPI (Sigma-Aldrich) was used to stain the nuclei. The cells were mounted using Vectashield (Vector Labs) and imaged using fluorescence microscopy.
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