Total proteins were extracted by RIPA lysis buffer (Beyotime Institute of Biotechnology), separated on SDS-PAGE gels and transferred to PVDF membranes (EMD Millipore, Billerica, MA, USA). Membranes were blocked with 5% nonfat milk in Tris-buffered saline with Tween-20, and then incubated with primary antibody overnight at 4 °C, and with corresponding secondary antibody (1:40 000; Catalogue No: SA00001-1, SA00001-2; ProteinTech, Chicago, IL, USA). The primary antibody being used are as follows: rabbit anti-human ISG15 (1:1000; Catalogue No: 15981-1-AP; ProteinTech), rabbit anti-human ZFP36 (1:1000; Catalogue No: 12737-1-AP; ProteinTech), mouse anti-human α-tublin (1:100,000; Catalogue No: 66031-1-Ig; ProteinTech). Protein bands were visualized with ECL (Beyotime Institute of Biotechnology).
Mouse anti human α tublin
Manufactured by Proteintech
Mouse anti-human α-tublin is a primary antibody used for the detection and analysis of α-tubulin, a key component of the cytoskeleton in human cells. This antibody is specific to the α-tubulin protein and can be used in various immunodetection techniques, such as Western blotting, immunofluorescence, and immunohistochemistry.
Automatically generated - may contain errors
2 protocols using mouse anti human α tublin
1
Immunoblotting Assay for Protein Expression
2
Western Blot Analysis of Cellular Proteins
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Total proteins were extracted by RIPA lysis buffer (Beyotime Institute of Biotechnology), separated on SDS-PAGE gels and transferred to PVDF membranes (EMD Millipore, Billerica, MA, USA). Membranes were blocked with 5% nonfat milk in Tris-buffered saline with Tween-20, and then incubated with primary antibody overnight at 4℃,and with corresponding secondary antibody (1:40 000; Catalogue No: SA00001-1, SA00001-2; ProteinTech, Chicago, IL, USA). The primary antibody being used are as follows: rabbit anti-human ISG15 (1:1000; Catalogue No: 15981-1-AP; ProteinTech), rabbit anti-human ZFP36 (1:1000; Catalogue No: 12737-1-AP; ProteinTech), mouse anti-human α-tublin(1:100000; Catalogue No: 66031-1-Ig; ProteinTech). Protein bands were visualized with ECL (Beyotime Institute of Biotechnology).
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