Total s6 antibody

Manufactured by Cell Signaling Technology

The Total S6 antibody is a tool used in research settings to detect and quantify the S6 protein, which is a key component of the ribosomal protein S6 and plays a role in cell growth and proliferation. This antibody provides a means to measure the total levels of S6 protein in cell and tissue samples, without making any claims about its intended use or interpretation of the results.

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Lab products found in correlation

Chloroquine c6628, by Merck Group (1 mentions) Beclin 1 antibody, by Cell Signaling Technology (1 mentions) Torin1 cay10997, by Cayman Chemical (1 mentions) Bafilomycin a1 b1793 2ug, by Merck Group (1 mentions) Lc 3 antibody nb100 2220, by Novus Biologicals (1 mentions) Phospho thr389 p70s6k sc 11759, by Santa Cruz Biotechnology (1 mentions) Total ulk1 antibody, by Cell Signaling Technology (1 mentions) β tubulin, by Merck Group (1 mentions) β actin antibody, by Merck Group (1 mentions) Rabbit anti phospho s6 antibody, by Cell Signaling Technology (1 mentions) Goat anti actin polyclonal antibody, by Santa Cruz Biotechnology (1 mentions) Goat anti type1 collagen antibody, by Southern Biotech (1 mentions) Ecl plu, by Cytiva (1 mentions) Enhanced chemi luminescence (ecl), by Cytiva (1 mentions)

Close spelling variants of this product

Total S6

2 protocols using total s6 antibody

Evaluation of LRRK2 and VPS34 Inhibitors

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The LRRK2in1 compound and the VPS34in1 were purchased from the Division of Signal Transduction Therapy, School of Life Sciences, and University of Dundee, UK. The GSK2578215A compound was purchased from Tocris. Bafilomycin A1 (B1793-2UG), Chloroquine (C6628) and Wortmannin (W3144-250UL) were purchased from Sigma-Aldrich. Torin-1 (CAY10997) was purchased from Cayman Chemicals.
Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total S6 antibody (2317, Cell Signalling); phospho Ser235/236 S6 antibody (2211S, Cell Signalling); total P70S6K antibody (sc-8418, Santa Cruz); phospho Thr389 P70S6K (sc-11759, Santa Cruz); total ULK1 antibody (8054 and 4773, Cell Signalling); phospho Ser757 ULK1 antibody (6888, Cell Signalling); Beclin-1 antibody (3738, Cell Signalling); β-actin antibody (A1978, Sigma Aldrich); β-tubulin (T6199, Sigma Aldrich).

Manzoni C., Mamais A., Roosen D.A., Dihanich S., Soutar M.P., Plun-Favreau H., Bandopadhyay R., Hardy J., Tooze S.A., Cookson M.R, & Lewis P.A. (2016). mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1. Scientific Reports, 6, 35106.

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Western Blot Analysis of Collagen and S6 Phosphorylation

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Western blot analysis was performed on proteins from tissue homogenates and cell lysates as previously reported.4, 6, 18, 19 Goat anti‐type 1 collagen antibody (Southern Biotech, Birmingham, AL) was used to probe for collagen‐1, and secondary anti‐goat antibody was purchased from Santa Cruz Biotechnology (Dallas, TX). Rabbit anti‐phospho‐S6 antibody and total S‐6 antibody were purchased from Cell Signaling Technology (Danvers, MA). For detection, we used ECL and ECL plus purchased from Amersham Biosciences (Foster City, CA). Loading conditions were controlled using anti‐actin goat polyclonal antibody (Santa Cruz Biotechnology).

Haller S.T., Yan Y., Drummond C.A., Xie J., Tian J., Kennedy D.J., Shilova V.Y., Xie Z., Liu J., Cooper C.J., Malhotra D., Shapiro J.I., Fedorova O.V, & Bagrov A.Y. (2016). Rapamycin Attenuates Cardiac Fibrosis in Experimental Uremic Cardiomyopathy by Reducing Marinobufagenin Levels and Inhibiting Downstream Pro‐Fibrotic Signaling. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(10), e004106.

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