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Basal media eagle media

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Basal Media Eagle media is a cell culture media formulation developed for the growth and maintenance of a variety of cell lines. It provides the necessary nutrients and components to support basic cellular functions. The exact composition and specifications of the media can be obtained from the product documentation.

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Anhydrous dmso, by Merck Group (2 mentions)

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Basal Media Eagle (2 citations)

2 protocols using basal media eagle media

1

Preparation of Aβ Oligomers for Cell Culture Studies

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Synthetic amyloid beta (Aβ) 1–42 was purchased from California Peptide (catalog number 641 15). An Aβ monomer film was prepared by evaporating the 1,1,1,3,3,3,hexafluoro-2-propanol (HFIP) (Sigma-Aldrich, St. Louis, Missouri, USA; catalog number 105,228) at room temperature from a solution of 253 μg Aβ(1–42) in HFIP at room temperature for 20 min using N2 gas. The film was then dissolved in 10.12 μL anhydrous DMSO (Sigma-Aldrich; catalog number D2650) to make a 5 mM working solution. This solution was diluted to 100 μM with cold Basal Media Eagle media (Life Technologies; catalog number 21,010), followed by incubation at 4 °C for 24 h to form oligomers. The resulting oligomer preparations were centrifuged at 16,000 × g to pellet any insoluble fibrils and the supernatant was diluted in Neurobasal media prior to addition to cultures.
LaBarbera K.M., Limegrover C., Rehak C., Yurko R., Izzo N.J., Knezovich N., Watto E., Waybright L, & Catalano S.M. (2021). Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery. Journal of neuroscience methods, 358, 109180.
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2

Preparation of Aβ Oligomers for Cell Culture Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Synthetic amyloid beta (Aβ) 1–42 was purchased from California Peptide (catalog number 641 15). An Aβ monomer film was prepared by evaporating the 1,1,1,3,3,3,hexafluoro-2-propanol (HFIP) (Sigma-Aldrich, St. Louis, Missouri, USA; catalog number 105,228) at room temperature from a solution of 253 μg Aβ(1–42) in HFIP at room temperature for 20 min using N2 gas. The film was then dissolved in 10.12 μL anhydrous DMSO (Sigma-Aldrich; catalog number D2650) to make a 5 mM working solution. This solution was diluted to 100 μM with cold Basal Media Eagle media (Life Technologies; catalog number 21,010), followed by incubation at 4 °C for 24 h to form oligomers. The resulting oligomer preparations were centrifuged at 16,000 × g to pellet any insoluble fibrils and the supernatant was diluted in Neurobasal media prior to addition to cultures.
LaBarbera K.M., Limegrover C., Rehak C., Yurko R., Izzo N.J., Knezovich N., Watto E., Waybright L, & Catalano S.M. (2021). Modeling the mature CNS: A predictive screening platform for neurodegenerative disease drug discovery. Journal of neuroscience methods, 358, 109180.
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