Mouse anti c src

Western blotting was performed as previously described [29 (link)], using the following antibodies: mouse anti-Cbl-b, mouse anti-c-Src, and rabbit anti-β-actin antibodies were obtained from Santa Cruz Biotechnology; rabbit anti-Akt, anti-p-Akt (Ser473), anti-ERK1/2, anti-p-ERK1/2 (Thr202/Tyr204) and anti-p-Src (Y416) antibodies were obtained from Cell Signaling Technology, mouse anti-RANK antibodies were obtained from R&D, USA. Proteins were visualized using the enhanced chemiluminescence reagent (SuperSignal Western Pico Chemiluminescent Substrate; Pierce, Rockford, IL, USA) and signals were quantitated using NIH Image J software.

Immunoblot analysis was performed as previously described^{8 (link),35 (link)}, using rabbit anti-TFF3 polyclonal antibody. Mouse anti-β-actin, mouse anti-CDKN1A, mouse anti-BCL2, mouse anti-CDK2, rabbit anti-CDK4, rabbit anti-CCNE1, mouse anti-cSRC, and mouse anti-CCND1 antibody was obtained from Santa Cruz Biotechnology, CA. Rabbit anti-p-cSRC antibody were obtained from Cell Signaling, USA. Rabbit anti-pSTAT3(Tyr705) and mouse anti-STAT3 antibodies were obtained from Abcam, Cambridge, MA. Confocal microscopy scanning was performed as previously described^{51 (link)}. Secondary antibody, Alexa Fluor 488 goat anti-rabbit IgG was purchased from Invitrogen, Singapore. Rhodamine-conjugated phalloidin (Sigma, St Louis, MO) was used to visualize f-actin filaments.

Immunoblot analysis was performed as previously described [23 (link)], using rabbit anti-TFF3 antibody [14 (link)]. Mouse anti-β-ACTIN, rabbit anti-p-c-SRC, mouse anti-c-SRC, and mouse anti-γ-CTNNG antibody was obtained from Santa Cruz Biotechnology, Santa Cruz, CA, USA. Mouse anti-CDH1, mouse anti-CDH2, rabbit anti-OCLN, mouse anti-VIM, mouse anti- ITGA6, rabbit anti-pSTAT3, and mouse anti-STAT3 antibody was obtained from Abcam, Cambridge, MA, USA. Cell extracts were resolved by SDS-PAGE and immunoblotted, with the respective antibodies, as previously described [23 (link)]. β-ACTIN was used as input control for cell lysate. The sizes of detected protein bands in kDa are shown on the left side.
Confocal laser scanning microscopy was performed as previously described [22 (link)]. Nuclei were visualised with VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA). Images were captured using a confocal microscope (Eclipse C1 Plus Confocal; Nikon Instrument Inc., Melville, NY, USA). Primary antibody, Mouse anti-CDH1, and mouse anti-VIM was obtained from Abcam. Secondary antibody, Alexa Fluor 488 rabbit anti-mouse immunoglobulin (Ig)G and Alexa Fluor 568 rabbit anti-mouse IgG was obtained from Invitrogen, Singapore. Rhodamine-conjugated phalloidin (Sigma-Aldrich, St Louis, MO, USA) was used to visualize f-actin filaments.