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2 protocols using anti vangl2 n 13

1

Characterization of Vangl2 and Wnt Signaling

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HEK293T cells were from ATCC, and T98G, U87-MG, A1207, and U251 cells were gifts from Dr. Paul Knoepfler. T98G, U87-MG and U251 cells were authenticated by Arizona Research Labs (http://uagc.arl.arizona.edu/). Antibodies employed were anti-Vangl2 N-13, anti-Muc4 P-20, anti-Rac1, anti-EGFR (1005) from Santa Cruz, anti-FLAG M2, anti-tubulin, anti-actin AC-15 from Sigma, anti-HA 12CA5 for Ub-HA from Roche, anti-Myc 9E10 from Calbiochem, anti-V5 from Invitrogen, anti-RhoA from BD Biosciences, anti-FLRF/RNF41/Nrdp1 from Bethyl Laboratories, anti-HA C29F4 for Fzd7-HA western blots, anti-Dvl2, anti-phospho-JNK (T183/Y185), anti-phospho-Erk (T202/Y204), anti-phospho-Akt (S473), anti-phospho-β-catenin (Ser33/37/Thr41), anti-phospho-β-catenin (Ser552), and anti-β-catenin from Cell Signaling.
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2

Characterization of Vangl2 and Wnt Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were from ATCC, and T98G, U87-MG, A1207, and U251 cells were gifts from Dr. Paul Knoepfler. T98G, U87-MG and U251 cells were authenticated by Arizona Research Labs (http://uagc.arl.arizona.edu/). Antibodies employed were anti-Vangl2 N-13, anti-Muc4 P-20, anti-Rac1, anti-EGFR (1005) from Santa Cruz, anti-FLAG M2, anti-tubulin, anti-actin AC-15 from Sigma, anti-HA 12CA5 for Ub-HA from Roche, anti-Myc 9E10 from Calbiochem, anti-V5 from Invitrogen, anti-RhoA from BD Biosciences, anti-FLRF/RNF41/Nrdp1 from Bethyl Laboratories, anti-HA C29F4 for Fzd7-HA western blots, anti-Dvl2, anti-phospho-JNK (T183/Y185), anti-phospho-Erk (T202/Y204), anti-phospho-Akt (S473), anti-phospho-β-catenin (Ser33/37/Thr41), anti-phospho-β-catenin (Ser552), and anti-β-catenin from Cell Signaling.
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