GFP-ATG8e transgenic seedlings were observed and photographed using a Zeiss Axio Imager.A2 upright microscope (Zeiss, Oberkochen, Germany) equipped with Zeiss Axiocam BW/color digital cameras and a GFP-specific filter at the Iowa State University Microscopy and Nanoimaging Facility. Cells within the root elongation zone were photographed and the number of autophagosomes in each image was counted and averaged from at least 10 images per sample [87 (link)]. For monodansylcadaverine (MDC) staining, Arabidopsis seedling roots were stained with MDC (Sigma-Aldrich, 30432) as described previously [88 (link)]. MDC-stained seedlings were observed and imaged with the same fluorescence microscopy system using a DAPI-specific filter.
Imager a2 upright microscope
Manufactured by Zeiss
Sourced in Germany
The Imager.A2 is an upright microscope designed for routine biological and materials research applications. It provides high-quality imaging capabilities with a stable and ergonomic design. The microscope is equipped with a range of features to support various observation techniques, including bright-field, phase-contrast, and polarization microscopy.
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Lab products found in correlation
2 protocols using imager a2 upright microscope
1
Visualizing Autophagy in Arabidopsis
2
Microscopic Analysis of Synchronized Nematode Populations
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Synchronous animal populations were obtained by two-hour egg lay. The synchronous populations were cultured at 15°, with daily transfer to fresh NGM plates beginning at the onset of egg laying. At each indicated developmental time point, animals were selected from the population to use for microscopy. Animals were paralyzed in 1 mM levamisole and mounted on 2% agarose pads with glass coverslips and immediately analyzed using a Zeiss Axio Imager A2 upright microscope and a 63x/1.4 objective. Animals were analyzed using 470/40 nm excitation and 525/50 nm emission wavelengths, and images were captured with 25 ms exposure using a Zeiss Axiocam 506 camera. Pixel density was analyzed with Image J. Puncta were quantified by manual count. Approximately 25 animals per condition were used, and three biological replicates of the experiment were performed.
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