Recombinant mouse m csf protein 416 ml

Manufactured by R&D Systems

Recombinant Mouse M-CSF Protein (416-ML) is a protein produced using recombinant DNA technology. It functions as a macrophage colony-stimulating factor, which is a cytokine that plays a role in the differentiation and proliferation of macrophages.

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Lab products found in correlation

Am16708, by Thermo Fisher Scientific (1 mentions) Inf γ, by Merck Group (1 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Recombinant mouse rankl 462 tec, by R&D Systems (1 mentions) Pierce protein a g plus agarose, by Thermo Fisher Scientific (1 mentions) Anti ha beads ip0010, by Merck Group (1 mentions) Anti flag beads f2426, by Merck Group (1 mentions)

2 protocols using recombinant mouse m csf protein 416 ml

Macrophage Polarization Modulation

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Bone marrow-derived macrophages (BMDMs) were extracted from 12-week-old C57 BL/6J mice. BMDMs were cultured in DMEM medium containing 10% FBS and 10 ng/ml Recombinant Mouse M-CSF Protein (416-ML, R&D), followed by treatment with DMY (50, 100 μmol/L) or control vehicle for one hour, then stimulated with 100 ng/ml LPS (Cat# L2880, Sigma-Aldrich) and 20 ng/ml INF-γ (IF005, Sigma-Aldrich) for 24 hours. Lipofectamine 3000 transfection reagent (L3000008, Invitrogen) was used for miRNA (miR10000142-1-5, miR20000142-1-5, RiboBio) and siRNA (AM16708, Thermo Fisher Scientific) transfection according to the manufacturers' instructions.

Yang Z., Li T., Wang C., Meng M., Tan S, & Chen L. (2023). Dihydromyricetin Inhibits M1 Macrophage Polarization in Atherosclerosis by Modulating miR-9-Mediated SIRT1/NF-κB Signaling Pathway. Mediators of Inflammation, 2023, 2547588.

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Antibodies and Protein Reagents for Cell Signaling

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The antibodies used in this study are listed in Supplementary Table 1. Recombinant mouse M-CSF protein (# 416-ML) and Recombinant mouse RANKL (462-TEC) were purchased from R&D systems. Peptides were from GL Biochem (Shanghai) LTD. Staurosporine (S1421), KN-93(S6787), U73122 (S8011), Saracatinib (S1006), LY3214996(S8534), SB203580 (S1076) were from Selleck.Puromycin (P8833), Pierce™ GST agarose (20211) and Pierce™ Protein A/G plus agarose (20423) were purchased from Thermo Fisher, USA. anti-HA-beads (IP0010) and Anti-FLAG-beads (F2426) were obtained from Sigma-Aldrich, USA.

Li Z., Yang X., Fu R., Wu Z., Xu S., Jiao J., Qian M., Zhang L., Wu C., Xie T., Yao J., Wu Z., Li W., Ma G., You Y., Chen Y., Zhang H.K., Cheng Y., Tang X., Wu P., Lian G., Wei H., Zhao J., Xu J., Ai L., Siwko S., Wang Y., Ding J., Song G., Luo J., Liu M, & Xiao J. (2024). Kisspeptin-10 binding to Gpr54 in osteoclasts prevents bone loss by activating Dusp18-mediated dephosphorylation of Src. Nature Communications, 15, 1300.

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