Fkhgal4

All fly stocks were reared at 25°C on standard flour/agar Drosophila media. The Gal4/UAS system [58 (link)] was used to drive the expression of transgenes at 29°C. The following strains were provided by the Bloomington Drosophila Stock Center (BDSC) or the Vienna Drosophila RNAi Center (VDRC): Df(3R)BSC503 (BDSC 25007); Df(3R)ED6332 (BDSC 24141); hdc⁴³(BDSC 64063); hdc⁵⁰(BDSC 64064); hdc^BG23007(BDSC 12410); UAS-hdc (BDSC 64056); hdc^RI(VDRC 104322); hdc^R2(VDRC 45069); hdc^R3(BDSC 30489); UAS-p35 (BDSC 5073); UAS-Rheb (BDSC 9689); UAS-Xbp1-EGFP (BDSC 60731); Xbp1^RI(BDSC 36755); PEK^RI(VDRC 110278); UAS-myristoylated-Tomato (BDSC 32222); UAS-CD8-GFP (BDSC 5137); UAS-src-GFP (BDSC 5429); UAS-myrRFP (BDSC 7138); hsp70-GFP (BDSC 51354); phmGal4 (BDSC 80577); fkhGal4 (BDSC 78060); apGal4 (BDSC 3041), UAS-GFPnls (BDSC 4776); TM3-cherry (BDSC 35524); puc-LacZ (BDSC 11173); The following strains are described in Flybase: nub-Gal4 [59 (link)]; Ci-Gal4 [60 (link)]; sal^E/PV [61 (link)]; amn^c651 [10 (link)]. Gal4 drivers were recombined to UAS fluorescent markers described here. UAS-SOD1::UAS-Cat recombined construct was kindly provided by F. Serras. phmGal4::YPetAtet [16 (link)] was kindly provided by X. Franch-Marro. w¹¹⁸ strain was used as control.

The following fly stocks were used in this study: CFD2 and TH_attP2,⁷⁰ (link) Snky-GFP,⁷¹ (link) GFP-LAMP,⁷² (link) GFP::TMF (this study, see below) αManII::GFP (this study, see below), GMAP_2.2.5, TMF_Δ19, golgin-84_ Δ13.3 (all three this study, see below), fkh-GAL4 (BDSC 78060), en-GAL4 (BDSC 1973), and YFP::pgant9 (⁷⁵ (link), Kyoto Stock Centre 115535). The following UAS-X-mito stocks were generated (this study, see ΦC31 transgenesis): UASt-BioID2-mito (II), UASt-BioID2-mito (III), UASt-g245-mito (II), UASt-g245-mito (III), UASt-TMF-mito (II), UASt-TMF-mito (III), UASt-g84-mito (II), UASt-g84-mito (III), UASt-GMAP-mito (II), UASt-GMAP-mito (III).