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Talon immobilized metal affinity chromatography

Manufactured by Takara Bio
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The Talon Immobilized Metal Affinity Chromatography (IMAC) is a laboratory equipment product from Takara Bio. It is designed for the purification of recombinant proteins containing a histidine tag. The core function of this product is to selectively bind and separate the target protein from a complex mixture through the interaction between the histidine tag and the immobilized metal ions.

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Lab products found in correlation

Cht chromatography, by Bio-Rad (2 mentions)

Close spelling variants of this product

TALON metal affinity chromatography (12 citations) Metal affinity chromatography (5 citations) TALON affinity chromatography (4 citations) TALON chromatography (2 citations)

2 protocols using talon immobilized metal affinity chromatography

1

Recombinant Antibody Expression in N. benthamiana

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Each of these molecules was expressed in N. benthamiana using the MagnICON® tobamoviral vector system. VRC01Fab and Avaren were each purified by a two-step purification scheme consisting of Talon Immobilized Metal Affinity Chromatography (IMAC) from Clontech followed by CHT chromatography from BioRad. Production of VRC01 and Avaren-Fc were done as described previously (Hamorsky et al., 2013 (link)).
Seber Kasinger L.E., Dent M.W., Mahajan G., Hamorsky K.T, & Matoba N. (2019). A novel anti‐HIV‐1 bispecific bNAb‐lectin fusion protein engineered in a plant‐based transient expression system. Plant Biotechnology Journal, 17(8), 1646-1656.
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2

Expression and Purification of VRC01 and Avaren

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Each of these molecules was expressed in N. benthamiana using the MagnICON® tobamoviral vector system. VRC01Fab and Avaren were each purified by a two‐step purification scheme consisting of Talon Immobilized Metal Affinity Chromatography (IMAC) from Clontech followed by CHT chromatography from BioRad. Production of VRC01 and Avaren‐Fc were done as described previously (Hamorsky et al., 2019; submitted).
Seber Kasinger L.E., Dent M.W., Mahajan G., Hamorsky K.T, & Matoba N. (2019). A novel anti‐HIV‐1 bispecific bNAb‐lectin fusion protein engineered in a plant‐based transient expression system. Plant Biotechnology Journal, 17(8), 1646-1656.
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