Ez1 dna blood 200 l kit

We collected buccal swap samples from 64 father-son pairs from European populations with written informed consent signed by all participants. Our analyses are based on self-declared family relations. DNA extraction was performed on the BioRobot® EZ1 (Qiagen, Hilden, Germany) using the EZ1 DNA Blood 200 µL Kit (Qiagen). Quantification of DNA samples was performed with the Rotor-Gene Q (Qiagen) and the Investigator Quantiplex Kit (Qiagen) or with the Quantus^™ Fluorometer (Promega Corporation, Madison, WI, USA) and the QuantiFluor^® ONE dsDNA Kit (Promega Corporation).

This study enrolled 1976 individuals, including 976 exudative-AMD cases and 1000 control subjects. Cases were selected from the Ophthalmology Unit of the PTV General Hospital of Rome, U.O. Oculist Foundation IRCCS “Cà Granda” Maggiore General Hospital of Milan, Department of Clinical Physiopathology of the University of Turin, and Department of Clinical Science of Sacco Hospital of Milan. The control subjects were recruited from the UOSD SIMT and the Ophthalmology Unit of the PTV General Hospital of Rome. Clinical data referred to the recruited subjects are summarized in Table 3. The study was approved by the Ethics Committee of the “Tor Vergata”, University of Rome (reference number: 16.15, approved on 23 January 2015). The study was performed according to the Declaration of Helsinki, and all participants provided signed informed consent. Blood samples were obtained from all subjects in order to extract genomic DNA. Genomic DNA extraction was performed using the EZ1 Advanced XL automated extractor and the EZ1 DNA Blood 200 µL Kit (Qiagen, Valencia, CA, USA) according to manufacturer’s instructions.